ManP

• Description: trigger enzyme: mannose-specific phosphotransferase system, EIIBCA of the PTS
  
  

Gene name	manP
Synonyms	yjdD
Essential	no
Product	trigger enzyme: mannose-specific phosphotransferase system, EIIBCA
Function	mannose uptake and phosphorylation, control of ManR activity
Gene expression levels in SubtiExpress: manP
Interactions involving this protein in SubtInteract: ManP
Metabolic function and regulation of this protein in SubtiPathways: manP
MW, pI	61 kDa, 6.715
Gene length, protein length	1767 bp, 589 aa
Immediate neighbours	manR, manA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, transcription factors and their control, trigger enzyme, membrane proteins, phosphoproteins

This gene is a member of the following regulons

ManR regulon

The gene

Basic information

• Locus tag: BSU12010

Database entries

• BsubCyc: BSU12010

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transport and concomitant phosphorylation of mannose

• Protein family: PTS permease, fructose/ mannitol permease (Fru) family PubMed

• Paralogous protein(s): FruA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-365 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ManP-ManR, ManP-HPr

• Localization: membrane

Database entries

• BsubCyc: BSU12010

• Structure: 2R48 (IIB domain)

• UniProt: O31645

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: manP-manA-yjdF PubMed

• Expression browser: manP PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by mannose (ManR) PubMed
  • repressed by glucose (ManR) PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • ManR: transcription activation in the presence of mannose and absence of glucose PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Marian Wenzel, Josef Altenbuchner
The Bacillus subtilis mannose regulator, ManR, a DNA-binding protein regulated by HPr and its cognate PTS transporter ManP.
Mol Microbiol: 2013, 88(3);562-76
[PubMed:23551403] [WorldCat.org] [DOI] (I p)

Imke G de Jong, Jan-Willem Veening, Oscar P Kuipers
Single cell analysis of gene expression patterns during carbon starvation in Bacillus subtilis reveals large phenotypic variation.
Environ Microbiol: 2012, 14(12);3110-21
[PubMed:23033921] [WorldCat.org] [DOI] (I p)

Tianqi Sun, Josef Altenbuchner
Characterization of a mannose utilization system in Bacillus subtilis.
J Bacteriol: 2010, 192(8);2128-39
[PubMed:20139185] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)