LipC

• Description: spore coat protein, phospholipase implicated in spore germination
  
  

Gene name	lipC
Synonyms	ycsK
Essential	no
Product	spore coat phospholipase B
Function	spore germination
Gene expression levels in SubtiExpress: lipC
Function and regulation of this protein in SubtiPathways: lipC
MW, pI	23 kDa, 5.839
Gene length, protein length	639 bp, 213 aa
Immediate neighbours	kipR, yczI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of lipids, sporulation proteins, germination

This gene is a member of the following regulons

KipR regulon, SigK regulon, TnrA regulon, GerE regulon

The gene

Basic information

• Locus tag: BSU04110

Phenotypes of a mutant

• defective in L-alanine-stimulated germination PubMed

Database entries

• BsubCyc: BSU04110

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: cleaves the fatty acids at the sn-1 and sn-2 positions of phospholipids PubMed

• Protein family: 'GDSL' lipolytic enzyme family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • spore coat (basement), localization depends on SpoIVA PubMed

Database entries

• BsubCyc: BSU04110

• Structure:

• UniProt: P42969

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • ycsF-ycsG-ycsI-kipI-kipA-kipR-lipC PubMed
  • lipC (according to DBTBS)

• Expression browser: lipC PubMed

• Sigma factor:
  • lipC: SigK PubMed

• Regulation:
  • lipC: expressed four hours after onset of sporulation (GerE) PubMed

• Regulatory mechanism:
  • ycsF: KipR: transcription repression PubMed
  • ycsF: TnrA: transcription activation PubMed
  • GerE: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Peter T McKenney, Adam Driks, Patrick Eichenberger
The Bacillus subtilis endospore: assembly and functions of the multilayered coat.
Nat Rev Microbiol: 2013, 11(1);33-44
[PubMed:23202530] [WorldCat.org] [DOI] (I p)

Original publications

Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814] [WorldCat.org] [DOI] (I p)

Atsushi Masayama, Shiro Kato, Takuya Terashima, Anne Mølgaard, Hisashi Hemmi, Tohru Yoshimura, Ryuichi Moriyama
Bacillus subtilis spore coat protein LipC is a phospholipase B.
Biosci Biotechnol Biochem: 2010, 74(1);24-30
[PubMed:20057119] [WorldCat.org] [DOI] (I p)

Atsushi Masayama, Ritsuko Kuwana, Hiromu Takamatsu, Hisashi Hemmi, Tohru Yoshimura, Kazuhito Watabe, Ryuichi Moriyama
A novel lipolytic enzyme, YcsK (LipC), located in the spore coat of Bacillus subtilis, is involved in spore germination.
J Bacteriol: 2007, 189(6);2369-75
[PubMed:17220230] [WorldCat.org] [DOI] (P p)

L Wang, R Grau, M Perego, J A Hoch
A novel histidine kinase inhibitor regulating development in Bacillus subtilis.
Genes Dev: 1997, 11(19);2569-79
[PubMed:9334321] [WorldCat.org] [DOI] (P p)