EfeB

• Description: elemental iron uptake system, heme peroxidase, converts ferrous iron (Fe(II) to ferric iron (FeIII)) for uptake by EfeO-EfeU, peroxide detoxification under microaerobic conditions
  
  

Gene name	efeB
Synonyms	ipa-29d, ywbN
Essential	no
Product	heme peroxidase in elemental iron uptake
Function	ferrous iron conversion
Gene expression levels in SubtiExpress: efeB
Interactions involving this protein in SubtInteract: EfeB
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	45 kDa, 8.64
Gene length, protein length	1248 bp, 416 aa
Immediate neighbours	ywbO, efeO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

acquisition of iron, iron metabolism, cell envelope stress proteins (controlled by SigM, V, W, X, Y), resistance against oxidative and electrophile stress, membrane proteins

This gene is a member of the following regulons

Fur regulon, SigM regulon, SigW regulon, SigX regulon

The gene

Basic information

• Locus tag: BSU38260

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • oxidizes ferrous iron to ferric iron for uptake by EfeO-EfeU PubMed
  • eliminates reactive oxygen species that accumulate in the presence of ferrous iron PubMed
  • peroxide detoxification under microaerobic conditions PubMed

• Protein family: DyP-type peroxidase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:
  • protoheme IX PubMed

• Effectors of protein activity:
  • activity is increased in the presence of EfeO due to the displacement of ferric iron PubMed

• Interactions:
  • EfeO-EfeU-EfeB PubMed

• Localization:
  • extracellular, secreted by the TatAY-TatCY complex PubMed
  • TatAY-TatCY-dependent export of EfeB requires a functional WprA PubMed
  • forms a membrane-bound complex with EfeU and EfeO for iron uptake PubMed
  • attached to the membrane via EfeU PubMed

Database entries

• Structure:

• UniProt: P39597

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • efeU-efeO-efeB PubMed
  • efeB-ywbO PubMed

• Expression browser: efeB PubMed

• Sigma factor:
  • efeU: SigA PubMed
  • efeB: SigM PubMed, SigX, SigW PubMed

• Regulation:
  • induced upon iron starvation (Fur) PubMed

• Regulatory mechanism:
  • Fur: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Jan Maarten van Dijl, Groningen, Netherlands

References

Reviews

Vivianne J Goosens, Carmine G Monteferrante, Jan Maarten van Dijl
The Tat system of Gram-positive bacteria.
Biochim Biophys Acta: 2014, 1843(8);1698-706
[PubMed:24140208] [WorldCat.org] [DOI] (P p)