Difference between revisions of "PurS"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[biosynthesis/ acquisition of nucleotides]]}} | + | {{SubtiWiki category|[[biosynthesis/ acquisition of nucleotides]]}}, |
| + | [[most abundant proteins]] | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''Additional information:''' | * '''Additional information:''' | ||
| + | ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}} | ||
=Biological materials = | =Biological materials = | ||
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=References= | =References= | ||
| − | <pubmed>15301532,15301530,10784038,3036807,,7638212, </pubmed> | + | <pubmed>15301532,15301530,10784038,3036807,,7638212, 15378759</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:41, 5 March 2014
- Description: phosphoribosylformylglycinamidine synthase
| Gene name | purS |
| Synonyms | yexA |
| Essential | no |
| Product | phosphoribosylformylglycinamidine synthase |
| Function | purine biosynthesis |
| Gene expression levels in SubtiExpress: purS | |
| Interactions involving this protein in SubtInteract: PurS | |
| Metabolic function and regulation of this protein in SubtiPathways: purS | |
| MW, pI | 9 kDa, 4.594 |
| Gene length, protein length | 252 bp, 84 aa |
| Immediate neighbours | purC, purQ |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
biosynthesis/ acquisition of nucleotides, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU06460
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: UPF0062 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- Structure: 1T4A
- UniProt: P12049
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- belongs to the 100 most abundant proteins PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759]
[WorldCat.org]
[DOI]
(P p)
Ruchi Anand, Aaron A Hoskins, Eric M Bennett, Michael D Sintchak, JoAnne Stubbe, Steven E Ealick
A model for the Bacillus subtilis formylglycinamide ribonucleotide amidotransferase multiprotein complex.
Biochemistry: 2004, 43(32);10343-52
[PubMed:15301532]
[WorldCat.org]
[DOI]
(P p)
Aaron A Hoskins, Ruchi Anand, Steven E Ealick, JoAnne Stubbe
The formylglycinamide ribonucleotide amidotransferase complex from Bacillus subtilis: metabolite-mediated complex formation.
Biochemistry: 2004, 43(32);10314-27
[PubMed:15301530]
[WorldCat.org]
[DOI]
(P p)
Hans H Saxild, Per Nygaard
The yexA gene product is required for phosphoribosylformylglycinamidine synthetase activity in Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 4);807-814
[PubMed:10784038]
[WorldCat.org]
[DOI]
(P p)
M Weng, P L Nagy, H Zalkin
Identification of the Bacillus subtilis pur operon repressor.
Proc Natl Acad Sci U S A: 1995, 92(16);7455-9
[PubMed:7638212]
[WorldCat.org]
[DOI]
(P p)
D J Ebbole, H Zalkin
Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis.
J Biol Chem: 1987, 262(17);8274-87
[PubMed:3036807]
[WorldCat.org]
(P p)
