Difference between revisions of "Sat"

From SubtiWiki
Jump to: navigation, search
Line 37: Line 37:
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
<br/><br/><br/><br/>
+
<br/><br/>
<br/><br/><br/><br/>
 
 
 
 
 
<br/><br/><br/><br/><br/><br/>
 
  
 
= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[sulfur metabolism]]}}
+
{{SubtiWiki category|[[sulfur metabolism]]}},
 +
[[most abundant proteins]]
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
Line 65: Line 62:
  
 
=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
Line 83: Line 77:
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
Line 113: Line 107:
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sat_1632208_1633356_1 sat] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sat_1632208_1633356_1 sat] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigA]] {{PubMed|11004190}}
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|11004190}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
Line 125: Line 119:
  
 
* '''Additional information:'''
 
* '''Additional information:'''
 +
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
  
 
=Biological materials =
 
=Biological materials =
Line 148: Line 143:
 
=References=
 
=References=
  
<pubmed>16267287,11004190,12107147, 18039762 </pubmed>
+
<pubmed>16267287,11004190,12107147, 18039762 15378759</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:16, 5 March 2014

  • Description: sulfate adenylyltransferase

Gene name sat
Synonyms ylnB
Essential no
Product sulfate adenylyltransferase
Function sulfate activation
Gene expression levels in SubtiExpress: sat
Metabolic function and regulation of this protein in SubtiPathways:
sat
MW, pI 42 kDa, 5.646
Gene length, protein length 1146 bp, 382 aa
Immediate neighbours cysP, cysC
Sequences Protein DNA DNA_with_flanks
Genetic context
Sat context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Sat expression.png















Categories containing this gene/protein

sulfur metabolism, most abundant proteins

This gene is a member of the following regulons

CymR regulon, S-box

The gene

Basic information

  • Locus tag: BSU15590

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + sulfate = diphosphate + adenylyl sulfate (according to Swiss-Prot)
  • Protein family: sulfate adenylyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s): YitA

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
    • repressed in the presence of cysteine (CymR)
    • induced by methionine starvation (S-box) PubMed
  • Regulatory mechanism:
    • S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
    • CymR: transcription repression

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France

Your additional remarks

References