Difference between revisions of "GlyQ"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
{{SubtiWiki category|[[translation]]}}, | {{SubtiWiki category|[[translation]]}}, | ||
| − | {{SubtiWiki category|[[essential genes]]}} | + | {{SubtiWiki category|[[essential genes]]}}, |
| + | [[most abundant proteins]] | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glyQ_2607762_2608649_-1 glyQ] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glyQ_2607762_2608649_-1 glyQ] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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* '''Additional information:''' | * '''Additional information:''' | ||
| + | ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}} | ||
=Biological materials = | =Biological materials = | ||
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=References= | =References= | ||
| − | <pubmed>15890195,15663928,19258532,15292140,16741230,12923262, </pubmed> | + | <pubmed>15890195,15663928,19258532,15292140,16741230,12923262, 15378759</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 14:37, 5 March 2014
- Description: glycyl-tRNA synthetase (alpha subunit)
| Gene name | glyQ |
| Synonyms | yqfJ |
| Essential | yes PubMed |
| Product | glycyl-tRNA synthetase (alpha subunit) |
| Function | translation |
| Gene expression levels in SubtiExpress: glyQ | |
| Interactions involving this protein in SubtInteract: GlyQ | |
| Metabolic function and regulation of this protein in SubtiPathways: glyQ | |
| MW, pI | 33 kDa, 4.8 |
| Gene length, protein length | 885 bp, 295 aa |
| Immediate neighbours | glyS, recO |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
translation, essential genes, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU25270
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly) (according to Swiss-Prot)
- Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure: 1J5W (from Thermotoga maritima, 55% identity, 72% similarity)
- UniProt: P54380
- KEGG entry: [3]
- E.C. number: 6.1.1.14
Additional information
Expression and regulation
- Regulatory mechanism:
- T-box: RNA switch, transcriptional antitermination PubMed
- Additional information:
- belongs to the 100 most abundant proteins PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532]
[WorldCat.org]
[DOI]
(I p)
Audrey R Nelson, Tina M Henkin, Paul F Agris
tRNA regulation of gene expression: interactions of an mRNA 5'-UTR with a regulatory tRNA.
RNA: 2006, 12(7);1254-61
[PubMed:16741230]
[WorldCat.org]
[DOI]
(P p)
Mary R Yousef, Frank J Grundy, Tina M Henkin
Structural transitions induced by the interaction between tRNA(Gly) and the Bacillus subtilis glyQS T box leader RNA.
J Mol Biol: 2005, 349(2);273-87
[PubMed:15890195]
[WorldCat.org]
[DOI]
(P p)
Frank J Grundy, Mary R Yousef, Tina M Henkin
Monitoring uncharged tRNA during transcription of the Bacillus subtilis glyQS gene.
J Mol Biol: 2005, 346(1);73-81
[PubMed:15663928]
[WorldCat.org]
[DOI]
(P p)
Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759]
[WorldCat.org]
[DOI]
(P p)
Frank J Grundy, Tina M Henkin
Kinetic analysis of tRNA-directed transcription antitermination of the Bacillus subtilis glyQS gene in vitro.
J Bacteriol: 2004, 186(16);5392-9
[PubMed:15292140]
[WorldCat.org]
[DOI]
(P p)
Mary R Yousef, Frank J Grundy, Tina M Henkin
tRNA requirements for glyQS antitermination: a new twist on tRNA.
RNA: 2003, 9(9);1148-56
[PubMed:12923262]
[WorldCat.org]
[DOI]
(P p)
