Difference between revisions of "MenD"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU30820 menD]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU30820 menD]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/menaquinone.html Menaquinone]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=menD menD]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 63 kDa, 5.655   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 63 kDa, 5.655   

Revision as of 11:31, 7 January 2014

  • Description: 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / 2-oxoglutarate decarboxylase

Gene name menD
Synonyms
Essential yes PubMed
Product 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
synthase / 2-oxoglutarate decarboxylase
Function biosynthesis of menaquinone
Gene expression levels in SubtiExpress: menD
Metabolic function and regulation of this protein in SubtiPathways:
menD
MW, pI 63 kDa, 5.655
Gene length, protein length 1740 bp, 580 aa
Immediate neighbours ytxM, menF
Sequences Protein DNA DNA_with_flanks
Genetic context
MenD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MenD expression.png




























Categories containing this gene/protein

biosynthesis of cofactors, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU30820

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2 (according to Swiss-Prot)
  • Protein family: MenD subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): thiamine pyrophosphate, divalent metal ion such as Mn(2+)) PubMed
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Alice Dawson, Minjiao Chen, Paul K Fyfe, Zhihong Guo, William N Hunter
Structure and reactivity of Bacillus subtilis MenD catalyzing the first committed step in menaquinone biosynthesis.
J Mol Biol: 2010, 401(2);253-64
[PubMed:20600129] [WorldCat.org] [DOI] (I p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)

X Qin, H W Taber
Transcriptional regulation of the Bacillus subtilis menp1 promoter.
J Bacteriol: 1996, 178(3);705-13
[PubMed:8550504] [WorldCat.org] [DOI] (P p)

J R Driscoll, H W Taber
Sequence organization and regulation of the Bacillus subtilis menBE operon.
J Bacteriol: 1992, 174(15);5063-71
[PubMed:1629163] [WorldCat.org] [DOI] (P p)