RibE

• Description: riboflavin synthase (alpha subunit)
  
  

Gene name	ribE
Synonyms	ribB
Essential	no
Product	riboflavin synthase (alpha subunit)
Function	riboflavin biosynthesis
Gene expression levels in SubtiExpress: ribE
Interactions involving this protein in SubtInteract: RibE
Metabolic function and regulation of this protein in SubtiPathways: ribE
MW, pI	23 kDa, 5.845
Gene length, protein length	645 bp, 215 aa
Immediate neighbours	ribA, ribD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

FMN-box

The gene

Basic information

• Locus tag: BSU23270

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • (RibE)3-(RibH)60, the lumazine synthase/riboflavin synthase complex PubMed

• Localization:

Database entries

• Structure: 1I8D (from E. coli, 36% identity, 59% similarity) PubMed, , 1RVV (the RibE)3-(RibH)60 lumazine synthase/riboflavin synthase complex) PubMed

• UniProt: P16440

• KEGG entry: [3]

• E.C. number: 2.5.1.9

Expression and regulation

• Operon: ribD-ribE-ribA-ribH-ribT PubMed

• Sigma factor: SigA PubMed

• Regulation: expressed in the absence of FMN (FMN-box) PubMed

• Regulatory mechanism: FMN-box: riboswitch, mediates termination/ antitermination control of the operon, in the absence of FMN: antitermination, in the presence of FMN: termination PubMed

• Additional information:

Biological materials

• Mutant: GP203 (erm), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: