LtaS

• Description: polyglycerolphosphate lipoteichoic acid synthase
  
  

Gene name	ltaS
Synonyms	yflE
Essential	no
Product	major lipoteichoic acid synthase
Function	biosynthesis of lipoteichoic acid
Gene expression levels in SubtiExpress: ltaS
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis
MW, pI	74 kDa, 5.202
Gene length, protein length	1947 bp, 649 aa
Immediate neighbours	nagP, yflD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, membrane proteins, phosphoproteins

The gene

Basic information

• Locus tag:

Phenotypes of a mutant

• induction of SigM and SigX activities PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: LTA synthase family (according to Swiss-Prot)

• Paralogous protein(s): YfnI, YqgS, YvgJ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on Thr-297 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane, at the septum of the cell PubMed
  • extracellular (signal peptide) PubMed

Database entries

• Structure: 2W8D

• UniProt: O34952

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ltaS (according to DBTBS)

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP1389 ltaS::aphA3, available in Jörg Stülke's lab
  • GP1396 ltaS::tet, available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Nathalie T Reichmann, Angelika Gründling
Location, synthesis and function of glycolipids and polyglycerolphosphate lipoteichoic acid in Gram-positive bacteria of the phylum Firmicutes.
FEMS Microbiol Lett: 2011, 319(2);97-105
[PubMed:21388439] [WorldCat.org] [DOI] (I p)

Iain C Sutcliffe
Priming and elongation: dissection of the lipoteichoic acid biosynthetic pathway in Gram-positive bacteria.
Mol Microbiol: 2011, 79(3);553-6
[PubMed:21255102] [WorldCat.org] [DOI] (I p)

Original publications

Michihiro Hashimoto, Takahiro Seki, Satoshi Matsuoka, Hiroshi Hara, Kei Asai, Yoshito Sadaie, Kouji Matsumoto
Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with defects in lipoteichoic acid synthesis.
Microbiology (Reading): 2013, 159(Pt 1);23-35
[PubMed:23103977] [WorldCat.org] [DOI] (I p)

Mirka E Wörmann, Rebecca M Corrigan, Peter J Simpson, Steve J Matthews, Angelika Gründling
Enzymatic activities and functional interdependencies of Bacillus subtilis lipoteichoic acid synthesis enzymes.
Mol Microbiol: 2011, 79(3);566-83
[PubMed:21255105] [WorldCat.org] [DOI] (I p)

Kathrin Schirner, Jon Marles-Wright, Richard J Lewis, Jeff Errington
Distinct and essential morphogenic functions for wall- and lipo-teichoic acids in Bacillus subtilis.
EMBO J: 2009, 28(7);830-42
[PubMed:19229300] [WorldCat.org] [DOI] (I p)

Duo Lu, Mirka E Wörmann, Xiaodong Zhang, Olaf Schneewind, Angelika Gründling, Paul S Freemont
Structure-based mechanism of lipoteichoic acid synthesis by Staphylococcus aureus LtaS.
Proc Natl Acad Sci U S A: 2009, 106(5);1584-9
[PubMed:19168632] [WorldCat.org] [DOI] (I p)

Angelika Gründling, Olaf Schneewind
Synthesis of glycerol phosphate lipoteichoic acid in Staphylococcus aureus.
Proc Natl Acad Sci U S A: 2007, 104(20);8478-83
[PubMed:17483484] [WorldCat.org] [DOI] (P p)

Ayako Nishibori, Jin Kusaka, Hiroshi Hara, Masato Umeda, Kouji Matsumoto
Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes.
J Bacteriol: 2005, 187(6);2163-74
[PubMed:15743965] [WorldCat.org] [DOI] (P p)