ManA

• Description: mannose-6-phosphate isomerase, required for proper cell wall synthesis
  
  

Gene name	manA
Synonyms	yjdE
Essential	no
Product	mannose-6-phosphate isomerase
Function	mannose utilization
Gene expression levels in SubtiExpress: manA
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	35 kDa, 5.279
Gene length, protein length	945 bp, 315 aa
Immediate neighbours	manP, yjdF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

ManR regulon

The gene

Basic information

• Locus tag: BSU12020

Phenotypes of a mutant

• cells lose the characteristic rod shape typical of wild type B. subtilis cells and instead appear as elongated spheres, which are significantly larger than normal PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31646

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: manP-manA-yjdF PubMed

• Expression browser: manA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by mannose (ManR) PubMed
  • repressed by glucose (ManR) PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • ManR: transcription activation in the presence of mannose and absence of glucose PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Imke G de Jong, Jan-Willem Veening, Oscar P Kuipers
Single cell analysis of gene expression patterns during carbon starvation in Bacillus subtilis reveals large phenotypic variation.
Environ Microbiol: 2012, 14(12);3110-21
[PubMed:23033921] [WorldCat.org] [DOI] (I p)

Maya Elbaz, Sigal Ben-Yehuda
The metabolic enzyme ManA reveals a link between cell wall integrity and chromosome morphology.
PLoS Genet: 2010, 6(9);e1001119
[PubMed:20862359] [WorldCat.org] [DOI] (I e)

Tianqi Sun, Josef Altenbuchner
Characterization of a mannose utilization system in Bacillus subtilis.
J Bacteriol: 2010, 192(8);2128-39
[PubMed:20139185] [WorldCat.org] [DOI] (I p)

M S Turner, J D Helmann
Mutations in multidrug efflux homologs, sugar isomerases, and antimicrobial biosynthesis genes differentially elevate activity of the sigma(X) and sigma(W) factors in Bacillus subtilis.
J Bacteriol: 2000, 182(18);5202-10
[PubMed:10960106] [WorldCat.org] [DOI] (P p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)