Difference between revisions of "LcfA"
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Revision as of 14:13, 16 May 2013
- Description: long chain acyl-CoA synthetase, involved in surfactin production
| Gene name | lcfA |
| Synonyms | |
| Essential | no |
| Product | long chain acyl-CoA synthetase |
| Function | fatty acid degradation |
| Gene expression levels in SubtiExpress: lcfA | |
| Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation | |
| MW, pI | 62 kDa, 6.119 |
| Gene length, protein length | 1680 bp, 560 aa |
| Immediate neighbours | fadR, yshE |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU28560
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed
- Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P94547
- KEGG entry: [3]
- E.C. number: 6.2.1.3
Additional information
Expression and regulation
- Regulation:
- subject to carbon catabolite repression (CcpA-HPr(Ser-P) PubMed
- induced by long chain acyl-CoA (C14 ... C20) (FadR) PubMed
- Regulatory mechanism:
- CcpA-HPr(Ser-P): transcription repression PubMed
- FadR: transcription repression PubMed
- Additional information:
Biological materials
- Mutant: available in Mohamed Marahiel's lab PubMed
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
