Difference between revisions of "AraA"
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|style="background:#ABCDEF;" align="center"|'''Function''' || arabinose utilization | |style="background:#ABCDEF;" align="center"|'''Function''' || arabinose utilization | ||
|- | |- | ||
− | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28800 araA] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]''' | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[araB]]'', ''[[abnA]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[araB]]'', ''[[abnA]]'' | ||
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− | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28800 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28800 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28800 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:araA_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:araA_context.gif]] |
Revision as of 13:32, 13 May 2013
- Description: L-arabinose isomerase
Gene name | araA |
Synonyms | |
Essential | no |
Product | L-arabinose isomerase |
Function | arabinose utilization |
Gene expression levels in SubtiExpress: araA | |
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism | |
MW, pI | 56 kDa, 5.474 |
Gene length, protein length | 1494 bp, 498 aa |
Immediate neighbours | araB, abnA |
Sequences | Protein DNA Advanced_DNA |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
utilization of specific carbon sources
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU28800
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: L-arabinose = L-ribulose (according to Swiss-Prot)
- Protein family: arabinose isomerase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: k (cat) of 14,504 min(-1) and k (cat)/K (m) of 121 min(-1) mM(-1) for L-arabinose PubMed
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: P94523
- KEGG entry: [3]
- E.C. number: 5.3.1.4
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Jin-Ha Kim, Ponnandy Prabhu, Marimuthu Jeya, Manish Kumar Tiwari, Hee-Jung Moon, Raushan Kumar Singh, Jung-Kul Lee
Characterization of an L-arabinose isomerase from Bacillus subtilis.
Appl Microbiol Biotechnol: 2010, 85(6);1839-47
[PubMed:19727704]
[WorldCat.org]
[DOI]
(I p)
Irina Saraiva Franco, Luís Jaime Mota, Cláudio Manuel Soares, Isabel de Sá-Nogueira
Functional domains of the Bacillus subtilis transcription factor AraR and identification of amino acids important for nucleoprotein complex assembly and effector binding.
J Bacteriol: 2006, 188(8);3024-36
[PubMed:16585763]
[WorldCat.org]
[DOI]
(P p)
José Manuel Inácio, Carla Costa, Isabel de Sá-Nogueira
Distinct molecular mechanisms involved in carbon catabolite repression of the arabinose regulon in Bacillus subtilis.
Microbiology (Reading): 2003, 149(Pt 9);2345-2355
[PubMed:12949161]
[WorldCat.org]
[DOI]
(P p)
L J Mota, L M Sarmento, I de Sá-Nogueira
Control of the arabinose regulon in Bacillus subtilis by AraR in vivo: crucial roles of operators, cooperativity, and DNA looping.
J Bacteriol: 2001, 183(14);4190-201
[PubMed:11418559]
[WorldCat.org]
[DOI]
(P p)
L J Mota, P Tavares, I Sá-Nogueira
Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis.
Mol Microbiol: 1999, 33(3);476-89
[PubMed:10417639]
[WorldCat.org]
[DOI]
(P p)
Isabel Sa-Nogueira, Teresa V Nogueira, Snia Soares, Hermnia de Lencastre
The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence, genetic organization and expression.
Microbiology (Reading): 1997, 143 ( Pt 3);957-969
[PubMed:9084180]
[WorldCat.org]
[DOI]
(P p)
I Sá-Nogueira, H de Lencastre
Cloning and characterization of araA, araB, and araD, the structural genes for L-arabinose utilization in Bacillus subtilis.
J Bacteriol: 1989, 171(7);4088-91
[PubMed:2500424]
[WorldCat.org]
[DOI]
(P p)