Difference between revisions of "YloS"
| Line 14: | Line 14: | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || thiamine salvage | |style="background:#ABCDEF;" align="center"|'''Function''' || thiamine salvage | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15800 yloS] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin]''' | ||
| Line 24: | Line 24: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rpe]]'', ''[[spoVM]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rpe]]'', ''[[spoVM]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15800 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15800 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15800 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:yloS_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:yloS_context.gif]] | ||
Revision as of 12:48, 13 May 2013
- Description: thiamine pyrophosphokinase
| Gene name | yloS |
| Synonyms | thiN |
| Essential | no |
| Product | thiamine pyrophosphokinase |
| Function | thiamine salvage |
| Gene expression levels in SubtiExpress: yloS | |
| Metabolic function and regulation of this protein in SubtiPathways: Thiamin | |
| MW, pI | 23 kDa, 5.216 |
| Gene length, protein length | 642 bp, 214 aa |
| Immediate neighbours | rpe, spoVM |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU15800
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + thiamine = AMP + thiamine diphosphate (according to Swiss-Prot)
- Protein family: thiamine pyrophosphokinase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 3LM8
- UniProt: O34664
- KEGG entry: [2]
- E.C. number: 2.7.6.2
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578]
[WorldCat.org]
[DOI]
(I p)
Original publications
Ghislain Schyns, Sébastien Potot, Yi Geng, Teresa M Barbosa, Adriano Henriques, John B Perkins
Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8127-36
[PubMed:16291685]
[WorldCat.org]
[DOI]
(P p)
Jonathan Melnick, Ewa Lis, Joo-Heon Park, Cynthia Kinsland, Hirotada Mori, Tomoya Baba, John Perkins, Ghislain Schyns, Olga Vassieva, Andrei Osterman, Tadhg P Begley
Identification of the two missing bacterial genes involved in thiamine salvage: thiamine pyrophosphokinase and thiamine kinase.
J Bacteriol: 2004, 186(11);3660-2
[PubMed:15150256]
[WorldCat.org]
[DOI]
(P p)
