Difference between revisions of "NtdA"

From SubtiWiki
Jump to: navigation, search
Line 14: Line 14:
 
|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of the antibiotic kanosamine
 
|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of the antibiotic kanosamine
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU10550 ntdA]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10550 ntdA]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 5.971   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 5.971   
Line 22: Line 22:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ntdB]]'', ''[[ntdR]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ntdB]]'', ''[[ntdR]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12895&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10550 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10550 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10550 Advanced_DNA]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yhjL_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yhjL_context.gif]]

Revision as of 12:29, 13 May 2013

  • Description: pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:glutamate aminotransferase

Gene name ntdA
Synonyms yhjL
Essential no
Product pyridoxal phosphate-dependent

3-oxo-glucose-6-phosphate:glutamate aminotransferase

Function synthesis of the antibiotic kanosamine
Gene expression levels in SubtiExpress: ntdA
MW, pI 49 kDa, 5.971
Gene length, protein length 1323 bp, 441 aa
Immediate neighbours ntdB, ntdR
Sequences Protein DNA Advanced_DNA
Genetic context
YhjL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NtdA expression.png















Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds

This gene is a member of the following regulons

NtdR regulon

The gene

Basic information

  • Locus tag: BSU10550

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • 3-oxo-d-glucose-6-phosphate + glutamate --> kanosamine-6-phosphate PubMed
  • Protein family: degT/dnrJ/eryC1 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induced by 3,3'-neotrehalosadiamine (NtdR) PubMed
  • Regulatory mechanism: NtdR: positive regulator PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Takashi Inaoka, Kozo Ochi
Activation of dormant secondary metabolism neotrehalosadiamine synthesis by an RNA polymerase mutation in Bacillus subtilis.
Biosci Biotechnol Biochem: 2011, 75(4);618-23
[PubMed:21512256] [WorldCat.org] [DOI] (I p)

Original publications