NtdA

• Description: pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:glutamate aminotransferase
  
  

Gene name	ntdA
Synonyms	yhjL
Essential	no
Product	pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:glutamate aminotransferase
Function	synthesis of the antibiotic kanosamine
Gene expression levels in SubtiExpress: ntdA
MW, pI	49 kDa, 5.971
Gene length, protein length	1323 bp, 441 aa
Immediate neighbours	ntdB, ntdR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds

This gene is a member of the following regulons

NtdR regulon

The gene

Basic information

• Locus tag: BSU10550

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • 3-oxo-d-glucose-6-phosphate + glutamate --> kanosamine-6-phosphate PubMed

• Protein family: degT/dnrJ/eryC1 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):
  • pyridoxalphosphate PubMed

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O07566

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ntdA-ntdB-ntdC-glcP PubMed

• Expression browser: ntdA PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by 3,3'-neotrehalosadiamine (NtdR) PubMed

• Regulatory mechanism: NtdR: positive regulator PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Takashi Inaoka, Kozo Ochi
Activation of dormant secondary metabolism neotrehalosadiamine synthesis by an RNA polymerase mutation in Bacillus subtilis.
Biosci Biotechnol Biochem: 2011, 75(4);618-23
[PubMed:21512256] [WorldCat.org] [DOI] (I p)

Original publications

Natasha D Vetter, David M Langill, Shazia Anjum, Julie Boisvert-Martel, Rajendra C Jagdhane, Egiroh Omene, Hongyan Zheng, Karin E van Straaten, Isaac Asiamah, Ed S Krol, David A R Sanders, David R J Palmer
A previously unrecognized kanosamine biosynthesis pathway in Bacillus subtilis.
J Am Chem Soc: 2013, 135(16);5970-3
[PubMed:23586652] [WorldCat.org] [DOI] (I p)

K E van Straaten, D M Langill, D R J Palmer, D A R Sanders
Purification, crystallization and preliminary X-ray analysis of NtdA, a putative pyridoxal phosphate-dependent aminotransferase from Bacillus subtilis.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2009, 65(Pt 4);426-9
[PubMed:19342798] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Takenori Satomura, Yasutaro Fujita, Kozo Ochi
Novel gene regulation mediated by overproduction of secondary metabolite neotrehalosadiamine in Bacillus subtilis.
FEMS Microbiol Lett: 2009, 291(2);151-6
[PubMed:19087206] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Kozo Ochi
Glucose uptake pathway-specific regulation of synthesis of neotrehalosadiamine, a novel autoinducer produced in Bacillus subtilis.
J Bacteriol: 2007, 189(1);65-75
[PubMed:17056753] [WorldCat.org] [DOI] (P p)

Takashi Inaoka, Kosaku Takahashi, Hiroshi Yada, Mitsuru Yoshida, Kozo Ochi
RNA polymerase mutation activates the production of a dormant antibiotic 3,3'-neotrehalosadiamine via an autoinduction mechanism in Bacillus subtilis.
J Biol Chem: 2004, 279(5);3885-92
[PubMed:14612444] [WorldCat.org] [DOI] (P p)