CotC

• Description: spore coat protein (outer)
  
  

Gene name	cotC
Synonyms
Essential	no
Product	spore coat protein (outer)
Function	resistance of the spore
Gene expression levels in SubtiExpress: cotC
Interactions involving this protein in SubtInteract: CotC
MW, pI	14 kDa, 9.558
Gene length, protein length	354 bp, 118 aa
Immediate neighbours	ynzK, tatAC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

GerE regulon, SigK regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU17700

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • CotC-CotU PubMed
  • CotE-CotC PubMed

• Localization:
  • outer spore coat, more abundant at the mother cell-distal pole of the forespore PubMed
  • the CotC-CotU complex assembles around the forming spore PubMed

Database entries

• Structure:

• UniProt: P07790

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotC (according to DBTBS)

• Expression browser: cotC PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK, GerE, SpoIIID) PubMed

• Regulatory mechanism:
  • GerE: transcription activation PubMed
  • SpoIIID: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1S103 ( cotC::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Application of CotZ for protein surface display

Additional publications: PubMed

Rachele Isticato, Donatella Scotto Di Mase, Emilia M F Mauriello, Maurilio De Felice, Ezio Ricca
Amino terminal fusion of heterologous proteins to CotC increases display efficiencies in the Bacillus subtilis spore system.
Biotechniques: 2007, 42(2);151-2, 154, 156
[PubMed:17373477] [WorldCat.org] [DOI] (P p)