Difference between revisions of "GlmM"

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(Biological materials)
(Biological materials)
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* '''Mutant:'''
 
* '''Mutant:'''
** GP1380 lacA::cdaR-Strep aphA3 glmM-3xFLAG ermC (based on [[pGP1460]] and [[pGP1087]]), available in [[Jörg Stülke]]'s lab
 
 
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
Line 133: Line 131:
 
**pGP1403: (expression of GlmM (S100A) in ''B. subtilis'' in [[pBQ200]]), available in [[Jörg Stülke]]'s lab
 
**pGP1403: (expression of GlmM (S100A) in ''B. subtilis'' in [[pBQ200]]), available in [[Jörg Stülke]]'s lab
 
**pGP1405: (expression, purification of GlmM (S100A) in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
 
**pGP1405: (expression, purification of GlmM (S100A) in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
+
 
 
* '''lacZ fusion:'''  
 
* '''lacZ fusion:'''  
 
** GP1340 (cat) based on [[pAC6]], available in [[Jörg Stülke]]'s lab
 
** GP1340 (cat) based on [[pAC6]], available in [[Jörg Stülke]]'s lab
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''
 +
** GP1382 glmM-gfp ermC (based on [[pGP1080]]), available in [[Jörg Stülke]]'s lab
  
* '''two-hybrid system:'''  
+
* '''two-hybrid system:'''
 +
**pGP2576(glmM in p25N), available in [[Jörg Stülke]]'s lab 
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 13:11, 18 December 2012

  • Description: phosphoglucosamine mutase, required for cell wall synthesis

Gene name glmM
Synonyms ybbT
Essential yes PubMed
Product phosphoglucosamine mutase, required for cell wall synthesis
Function cell wall synthesis
Gene expression levels in SubtiExpress: glmM
Metabolic function and regulation of this protein in SubtiPathways:
Murein recycling
MW, pI 48 kDa, 4.677
Gene length, protein length 1344 bp, 448 aa
Immediate neighbours cdaR, glmS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YbbT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GlmM expression.png















Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU01770

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate (according to Swiss-Prot)
  • Protein family: phosphohexose mutase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-100 PubMed, this is an autophosphorylation PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:

Biological materials

  • Mutant:
  • Expression vector:
    • pGP400: (expression in B. subtilis in pBQ200), available in Jörg Stülke's lab
    • pGP1401: (expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
    • pGP1402: (cloning vector for glmM (GlmM S100A) in E. coli, in pBlueskript KS), available in Jörg Stülke's lab
    • pGP1403: (expression of GlmM (S100A) in B. subtilis in pBQ200), available in Jörg Stülke's lab
    • pGP1405: (expression, purification of GlmM (S100A) in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed