Difference between revisions of "GlmM"
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* '''Mutant:''' | * '''Mutant:''' | ||
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* '''Expression vector:''' | * '''Expression vector:''' | ||
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**pGP1403: (expression of GlmM (S100A) in ''B. subtilis'' in [[pBQ200]]), available in [[Jörg Stülke]]'s lab | **pGP1403: (expression of GlmM (S100A) in ''B. subtilis'' in [[pBQ200]]), available in [[Jörg Stülke]]'s lab | ||
**pGP1405: (expression, purification of GlmM (S100A) in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab | **pGP1405: (expression, purification of GlmM (S100A) in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab | ||
| − | + | ||
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
** GP1340 (cat) based on [[pAC6]], available in [[Jörg Stülke]]'s lab | ** GP1340 (cat) based on [[pAC6]], available in [[Jörg Stülke]]'s lab | ||
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
| + | ** GP1382 glmM-gfp ermC (based on [[pGP1080]]), available in [[Jörg Stülke]]'s lab | ||
| − | * '''two-hybrid system:''' | + | * '''two-hybrid system:''' |
| + | **pGP2576(glmM in p25N), available in [[Jörg Stülke]]'s lab | ||
* '''Antibody:''' | * '''Antibody:''' | ||
Revision as of 13:11, 18 December 2012
- Description: phosphoglucosamine mutase, required for cell wall synthesis
| Gene name | glmM |
| Synonyms | ybbT |
| Essential | yes PubMed |
| Product | phosphoglucosamine mutase, required for cell wall synthesis |
| Function | cell wall synthesis |
| Gene expression levels in SubtiExpress: glmM | |
| Metabolic function and regulation of this protein in SubtiPathways: Murein recycling | |
| MW, pI | 48 kDa, 4.677 |
| Gene length, protein length | 1344 bp, 448 aa |
| Immediate neighbours | cdaR, glmS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
cell wall synthesis, biosynthesis of cell wall components, essential genes, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU01770
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate (according to Swiss-Prot)
- Protein family: phosphohexose mutase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: O34824
- KEGG entry: [2]
- E.C. number: 5.4.2.10
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
Biological materials
- Mutant:
- Expression vector:
- pGP400: (expression in B. subtilis in pBQ200), available in Jörg Stülke's lab
- pGP1401: (expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
- pGP1402: (cloning vector for glmM (GlmM S100A) in E. coli, in pBlueskript KS), available in Jörg Stülke's lab
- pGP1403: (expression of GlmM (S100A) in B. subtilis in pBQ200), available in Jörg Stülke's lab
- pGP1405: (expression, purification of GlmM (S100A) in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
- lacZ fusion:
- GP1340 (cat) based on pAC6, available in Jörg Stülke's lab
- GFP fusion:
- GP1382 glmM-gfp ermC (based on pGP1080), available in Jörg Stülke's lab
- two-hybrid system:
- pGP2576(glmM in p25N), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
