FadN

• Description: 3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)
  
  

Gene name	fadN
Synonyms	yusL
Essential	no
Product	3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)
Function	fatty acid degradation
Gene expression levels in SubtiExpress: fadN
Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation
MW, pI	89 kDa, 6.53
Gene length, protein length	2445 bp, 815 aa
Immediate neighbours	fadA, fadM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of lipids

This gene is a member of the following regulons

CcpA regulon, FadR regulon, SdpR regulon

The gene

Basic information

• Locus tag: BSU32840

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-3-hydroxyacyl-CoA + NAD⁺ = 3-oxoacyl-CoA + NADH (according to Swiss-Prot)

• Protein family: 3-hydroxyacyl-CoA dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O32178

• KEGG entry: [3]

• E.C. number: 1.1.1.35

Expression and regulation

• Operon:
  • fadM-fadN-fadA-fadE PubMed
  • fadN-fadA-fadE PubMed

• Expression browser: fadN PubMed

• Sigma factor:
  • fadN: SigA PubMed

• Regulation:
  • fadN: induced by long-chain fatty acids (FadR) PubMed
  • fadN: subject to carbon catabolite repression (CcpA-HPr(Ser-P) PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed
  • SdpR: transcription activation PubMed
  • CcpA-HPr(Ser-P): transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

José E González-Pastor, Errett C Hobbs, Richard Losick
Cannibalism by sporulating bacteria.
Science: 2003, 301(5632);510-3
[PubMed:12817086] [WorldCat.org] [DOI] (I p)