Difference between revisions of "SrtN"

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* '''Mutant:'''
 
* '''Mutant:'''
** GP1210 (''[[srtN]]''::''cat''), available in [[Stülke]] lab
+
** GP1210 (''[[srtN]]''::''cat''), available in [[Jörg Stülke]]'s lab
** GP1213 (''[[srtN]]''::''cat'')(''[[acuC]]''::''kan''), available in [[Stülke]] lab** 1A888 ( ''srtN''::''spec''), {{PubMed|19136592}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A888&Search=1A888 BGSC]
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** 1A888 ( ''srtN''::''spec''), {{PubMed|19136592}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A888&Search=1A888 BGSC]
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 12:15, 1 October 2012

  • Description: protein deacetylase for the control of AcsA activity

Gene name srtN
Synonyms yhdZ
Essential no
Product NAD(+)-dependent AcsA deacetylase
Function control of AcsA activity
Gene expression levels in SubtiExpress: srtN
MW, pI 27 kDa, 5.134
Gene length, protein length 741 bp, 247 aa
Immediate neighbours yhdY, yheN
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YhdZ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SrtN expression.png















Categories containing this gene/protein

protein modification

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU09650

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein (according to Swiss-Prot) deacetylates (and thereby activates) AcsA (together with AcuC) PubMed
  • Protein family: deacetylase sirtuin-type domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jeffrey G Gardner, Jorge C Escalante-Semerena
In Bacillus subtilis, the sirtuin protein deacetylase, encoded by the srtN gene (formerly yhdZ), and functions encoded by the acuABC genes control the activity of acetyl coenzyme A synthetase.
J Bacteriol: 2009, 191(6);1749-55
[PubMed:19136592] [WorldCat.org] [DOI] (I p)