yheN

168

putative polysaccharide deacetylase

Locus: BSU_09660

Molecular weight: 31.58 kDa

Isoelectric point: 8.61

Protein length: 282 aa

Gene length: 849 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yheN

Genomic Context

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List of homologs in different organisms, belongs to COG0726 (Galperin et al., 2021)

Gene

Coordinates: 1,040,861  1,041,709

The protein

Protein family

• polysaccharide deacetylase family (according to UniProt)

Domains

• NodB homology domain (aa 85-271) (according to UniProt)

Structure

• 2C1G (PDB) (the NodB domain, from Streptococcus pneumoniae, 37% identity) PubMed
• O07596 (AlphaFold)

Paralogous protein(s)

• PdaC, (33%)

Expression and Regulation

Operons

• Genes: yheN

  Description: PubMed

Biological materials

Mutant

• MGNA-A708 (yheN::erm), available at the NBRP B. subtilis, Japan
• BKE09660 (yheN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAACAATCTCTCCATTGG,  downstream forward: _UP4_TAAAAAAGTGAGGCGCATAA
• BKK09660 (yheN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAACAATCTCTCCATTGG,  downstream forward: _UP4_TAAAAAAGTGAGGCGCATAA

References

Research papers

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