NadF

• Description: NAD kinase
  
  

Gene name	nadF
Synonyms	yjbN, ppnK
Essential	yes PubMed
Product	inorganic polyphosphate/ATP-NAD kinase
Function	generation of NADP from NAD
Gene expression levels in SubtiExpress: nadF
MW, pI	29 kDa, 5.991
Gene length, protein length	798 bp, 266 aa
Immediate neighbours	yjbM, yjbO
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, essential genes

The gene

Basic information

• Locus tag: BSU11610

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + NAD⁺ = ADP + NADP⁺ (according to Swiss-Prot)

• Protein family: NAD kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: allosterically activated by quinolinic acid

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O31612

• KEGG entry: [2]

• E.C. number: 2.7.1.23

Expression and regulation

• Operon: yjbL-yjbM-nadF-yjbO PubMed

• Expression browser: nadF PubMed

• Sigma factor:

• Regulation: expressed during exponential growth

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A825 ( nadF::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hideaki Nanamiya, Koji Kasai, Akira Nozawa, Choong-Soo Yun, Takakuni Narisawa, Kana Murakami, Yousuke Natori, Fujio Kawamura, Yuzuru Tozawa
Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis.
Mol Microbiol: 2008, 67(2);291-304
[PubMed:18067544] [WorldCat.org] [DOI] (P p)

Silvia Garavaglia, Alessandro Galizzi, Menico Rizzi
Allosteric regulation of Bacillus subtilis NAD kinase by quinolinic acid.
J Bacteriol: 2003, 185(16);4844-50
[PubMed:12897004] [WorldCat.org] [DOI] (P p)