Difference between revisions of "NadE"

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* '''Locus tag:''' BSU03130
 
* '''Locus tag:''' BSU03130
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[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nadE_338288_339106_1 Expression]
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===

Revision as of 13:23, 24 January 2012

  • Description: NH3-dependent NAD+ synthetase

Gene name nadE
Synonyms outB, tscBGH, gsp-81
Essential yes PubMed
Product NH3-dependent NAD+ synthetase
Function NAD biosynthesis
MW, pI 30 kDa, 4.888
Gene length, protein length 816 bp, 272 aa
Immediate neighbours ycgI, tmrB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NadE context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

biosynthesis of cofactors, general stress proteins (controlled by SigB), essential genes

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

  • Locus tag: BSU03130

Expression

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+ (according to Swiss-Prot)
  • Protein family: NAD synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References