Difference between revisions of "RapF"

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(Extended information on the protein)
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* '''Description:''' [[response regulator aspartate phosphatase]], controls [[ComA]] activity <br/><br/>
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* '''Description:''' [[response regulator aspartate phosphatase]], anti-activator protein, controls [[ComA]] activity <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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=== Additional information===
 
=== Additional information===
 
 
  
  
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* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
  
* '''Effectors of protein activity:''' binding of [[PhrF]] to [[RapF]] prevents it from binding [[ComA]] {{PubMed|15968044}}
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* '''Effectors of protein activity:''' binding of [[PhrF]] to [[RapF]] results in a conformational change of [[RapF]] and prevents it from binding [[ComA]] {{PubMed|15968044,22215984}}
  
 
* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
** [[RapF]]-[[ComA]] {{PubMed|15968044}}
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** [[RapF]]-[[ComA]] {{PubMed|15968044,22215984}}
** [[RapF]]-[[PhrF]] {{PubMed|15968044}}
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** [[RapF]]-[[PhrF]] {{PubMed|15968044,22215984}}
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
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* '''Structure:'''
 
* '''Structure:'''
 +
** [http://www.rcsb.org/pdb/explore.do?structureId=3ULQ 3ULQ] (structure of the complex between the DNA-binding C-terminal domain of [[ComA]] with [[RapF]]) {{PubMed|22215984}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P71002 P71002]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P71002 P71002]
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=References=
 
=References=
  
<pubmed>12850135, 15968044 11466295 16091051 </pubmed>
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<pubmed>12850135, 15968044 11466295 16091051 22215984</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 09:04, 5 January 2012

Gene name rapF
Synonyms ywhJ
Essential no
Product response regulator aspartate phosphatase
Function control of ComA activity
Interactions involving this protein in SubtInteract: RapF
MW, pI 45 kDa, 5.18
Gene length, protein length 1143 bp, 381 aa
Immediate neighbours ywhK, phrF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RapF context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

genetic competence, protein modification, transcription factors and their control, quorum sensing

This gene is a member of the following regulons

CcpA regulon, ComA regulon

The gene

Basic information

  • Locus tag: BSU37460

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: control of ComA activity PubMed
  • Protein family: RAP family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: binding of PhrF to RapF results in a conformational change of RapF and prevents it from binding ComA PubMed

Database entries

  • Structure:
    • 3ULQ (structure of the complex between the DNA-binding C-terminal domain of ComA with RapF) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: repressed by glucose (5-fold) (CcpA) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References