ComC

• Description: late competence gene required for processing and translocation of ComGC, ComGD, ComGE and ComGG PubMed
  
  

Gene name	comC
Synonyms
Essential	no
Product	processing protease
Function	genetic competence
Interactions involving this protein in SubtInteract: ComC
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	26 kDa, 9.843
Gene length, protein length	744 bp, 248 aa
Immediate neighbours	spoIIB, folC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

genetic competence, protein secretion, membrane proteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU28070

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • processing and translocation of ComGC, ComGD, ComGE and ComGG PubMed
  • Typically cleaves a -Gly-|-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine (according to Swiss-Prot)

• Protein family: peptidase A24 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ComC-ComGC PubMed
  • ComC-ComGD PubMed
  • ComC-ComGE PubMed
  • ComC-ComGG PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P15378

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: comC PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Inês Chen, David Dubnau
DNA uptake during bacterial transformation.
Nat Rev Microbiol: 2004, 2(3);241-9
[PubMed:15083159] [WorldCat.org] [DOI] (P p)

Original publications

Inês Chen, Roberta Provvedi, David Dubnau
A macromolecular complex formed by a pilin-like protein in competent Bacillus subtilis.
J Biol Chem: 2006, 281(31);21720-21727
[PubMed:16751195] [WorldCat.org] [DOI] (P p)

Y S Chung, F Breidt, D Dubnau
Cell surface localization and processing of the ComG proteins, required for DNA binding during transformation of Bacillus subtilis.
Mol Microbiol: 1998, 29(3);905-13
[PubMed:9723928] [WorldCat.org] [DOI] (P p)

Y S Chung, D Dubnau
ComC is required for the processing and translocation of comGC, a pilin-like competence protein of Bacillus subtilis.
Mol Microbiol: 1995, 15(3);543-51
[PubMed:7783624] [WorldCat.org] [DOI] (P p)

D van Sinderen, A ten Berge, B J Hayema, L Hamoen, G Venema
Molecular cloning and sequence of comK, a gene required for genetic competence in Bacillus subtilis.
Mol Microbiol: 1994, 11(4);695-703
[PubMed:8196543] [WorldCat.org] [DOI] (P p)

S Mohan, D Dubnau
Transcriptional regulation of comC: evidence for a competence-specific transcription factor in Bacillus subtilis.
J Bacteriol: 1990, 172(7);4064-71
[PubMed:1694528] [WorldCat.org] [DOI] (P p)

S Mohan, J Aghion, N Guillen, D Dubnau
Molecular cloning and characterization of comC, a late competence gene of Bacillus subtilis.
J Bacteriol: 1989, 171(11);6043-51
[PubMed:2553669] [WorldCat.org] [DOI] (P p)