AhpF

• Description: alkyl hydroperoxide reductase (large subunit) / NADH dehydrogenase
  
  

Gene name	ahpF
Synonyms	ndh
Essential	no
Product	alkyl hydroperoxide reductase (large subunit) / NADH dehydrogenase
Function	resistance against peroxide stres
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	54 kDa, 4.705
Gene length, protein length	1527 bp, 509 aa
Immediate neighbours	ahpC, bglA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

resistance against oxidative and electrophile stress, membrane proteins, phosphoproteins

This gene is a member of the following regulons

PerR regulon

The gene

Basic information

• Locus tag: BSU40100

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: NADH + acceptor = NAD⁺ + reduced acceptor (according to Swiss-Prot)

• Protein family: class-II pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on (Ser-48 OR Ser-49) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1HYU (from Salmonella typhimurium, 52% identity, 69% similarity) PubMed

• UniProt: P42974

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ahpC-ahpF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by H2O2 (PerR) PubMed

• Regulatory mechanism:
  • PerR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: