bglA

168

6-phospho-beta-glucosidase

Locus: BSU_40110

Molecular weight: 54.67 kDa

Isoelectric point: 4.65

Protein length: 479 aa

Gene length: 1440 bp

Function: beta-glucoside utilization

Product: 6-phospho-beta-glucosidase)

Essential: no

E.C.: 3.2.1.86

Synonyms: bglA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2723 (Galperin et al., 2021)

Gene

Coordinates: 4,121,166  4,122,605

The protein

Catalyzed reaction/ biological activity

• 6-phospho-β-D-glucosyl-(1→4)-D-glucose + H₂O --> D-glucose + D-glucose 6-phosphate (according to UniProt)

Protein family

• glycosyl hydrolase 1 family (according to UniProt)

Structure

• 2XHY (PDB) (from E. coli, 66% identity) PubMed
• P42973 (AlphaFold)

Expression and Regulation

Operons

• Genes: yyzE-bglA

  Description: PubMed

Biological materials

Mutant

• BKE40110 (bglA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTCCCATGTTTAATT,  downstream forward: _UP4_TAAGAAGCGCTCGAATCGAG
• BKK40110 (bglA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTCCCATGTTTAATT,  downstream forward: _UP4_TAAGAAGCGCTCGAATCGAG

References

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