Difference between revisions of "SwrAA/1"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
 +
{{SubtiWiki category|[[biofilm formation]]}},
 
{{SubtiWiki category|[[motility and chemotaxis]]}},
 
{{SubtiWiki category|[[motility and chemotaxis]]}},
 
{{SubtiWiki category|[[pseudogenes]]}}
 
{{SubtiWiki category|[[pseudogenes]]}}
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=== Additional information===
 
=== Additional information===
  
This protein is functional in undomesticated strains of ''B.subtilis'' but not in laboratory strains, such as 168, because of a frameshift mutation. Therefore laboratory strains of ''B.subtilis'' are unable to swarm.
+
* This protein is functional in undomesticated strains of ''B.subtilis'' but not in laboratory strains, such as 168, because of a frameshift mutation. Therefore laboratory strains of ''B.subtilis'' are unable to swarm.
 
+
* The frameshift in strain 168 is caused by a single insertion of an adenine in the codon for Tyr-12 which leads to the premature truncation of the protein in residue 13. In addition, the C-terminal section of ''swrAA'' was predicted to be an ORF (''[[yvzD]]'') by the genome project.
The frameshift in strain 168 is caused by a single insertion of an adenine in the codon for Tyr-12 which leads to the premature truncation of the protein in residue 13. In addition, the C-terminal section of ''swrAA'' was predicted to be an ORF (''[[yvzD]]'') by the genome project.
+
* Correction of ''[[sfp]]'', ''[[epsC]]'','' [[swrAA]]'', and ''[[degQ]]'' as well as introduction of ''rapP'' from a plasmid present in NCIB3610 results in biofilm formation in ''B. subtilis'' 168 {{PubMed|21278284}}
 
 
  
  
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==Original publications==
 
==Original publications==
<pubmed>16357223,19389763,16030230,18567663,15066026,, 19389763 19749039 </pubmed>
+
<pubmed>16357223,19389763,16030230,18567663,15066026,, 19389763 19749039 21278284 </pubmed>
  
 
[[Category:Pseudogenes]]
 
[[Category:Pseudogenes]]

Revision as of 10:17, 1 February 2011

  • Description: control of motility, enhances sigD transcription

Gene name swrA
Synonyms yvzD, swrAA, ifm
Essential no
Product swarming motility protein
Function essential for swarming differentiation on solid surfaces
MW, pI 13 kDa, 8.4
Gene length, protein length 336 bp, 112 aa
Immediate neighbours minJ, ctpB
Gene sequence (+200bp) Protein sequence
Genetic context
YvzD context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

biofilm formation, motility and chemotaxis, pseudogenes

This gene is a member of the following regulons

The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

  • This protein is functional in undomesticated strains of B.subtilis but not in laboratory strains, such as 168, because of a frameshift mutation. Therefore laboratory strains of B.subtilis are unable to swarm.
  • The frameshift in strain 168 is caused by a single insertion of an adenine in the codon for Tyr-12 which leads to the premature truncation of the protein in residue 13. In addition, the C-terminal section of swrAA was predicted to be an ORF (yvzD) by the genome project.
  • Correction of sfp, epsC, swrAA, and degQ as well as introduction of rapP from a plasmid present in NCIB3610 results in biofilm formation in B. subtilis 168 PubMed


The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)


Original publications