PnpA

• Description: polynucleotide phosphorylase
  
  

Gene name	pnpA
Synonyms	comR
Essential
Product	polynucleotide phosphorylase (PNPase) (EC 2.7.7.8)
Function	necessary for competence development
MW, pI	77 kDa, 4.89
Gene length, protein length	2115 bp, 705 aa
Immediate neighbours
Gene sequence (+200bp)	Protein sequence
Genetic context

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: PnpA-RnjA, PnpA-PfkA, PnpA-Rny PubMed

• Localization:

Database entries

• Structure: 3CDI (protein from E. coli)

• Swiss prot entry:

• KEGG entry:

• E.C. number:

Additional information

required for the expression of late competence genes comG and comK, requirement bypassed by a mecA disruption; may be necessary for modification of the srfA transcript (stabilization or translation activation)

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

References

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed