YflL

• Description: acylphosphatase
  
  

Gene name	yflL
Synonyms
Essential	no
Product	acylphosphatase
Function	unknown
MW, pI	10 kDa, 8.666
Gene length, protein length	273 bp, 91 aa
Immediate neighbours	nos, yflK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU07640

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

poorly characterized/ putative enzymes

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: An acylphosphate + H₂O = a carboxylate + phosphate (according to Swiss-Prot)

• Protein family: acylphosphatase-like domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3BR8, 2FHM PubMed

• UniProt: O35031

• KEGG entry: [3]

• E.C. number: 3.6.1.7

Expression and regulation

• Operon: yflL (according to DBTBS)

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • yflL transcription occurs in antisense orientation as compared to the surrounding nos-yflK operon

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jicheng Hu, Dan Li, Xiao-Dong Su, Changwen Jin, Bin Xia
Solution structure and conformational heterogeneity of acylphosphatase from Bacillus subtilis.
FEBS Lett: 2010, 584(13);2852-6
[PubMed:20447399] [WorldCat.org] [DOI] (I p)