nos

168

nitric-oxide synthase

Locus: BSU_07630

Molecular weight: 38.59 kDa

Isoelectric point: 5.53

Protein length: 363 aa

Gene length: 1092 bp

Function: production of nitric oxide

Product: nitric-oxide synthase

Essential: no

Synonyms: nos, yflM

Genomic Context

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List of homologs in different organisms, belongs to COG4362 (Galperin et al., 2021)

Gene

Coordinates: 836,653 → 837,744

The protein

Catalyzed reaction/ biological activity

• NADPH-dependent oxidation of l-arginine into l-citrulline PubMed
• 2 L-arginine + 4 O₂ + 3 reduced [flavodoxin] --> 5 H⁺ + 4 H₂O + 2 L-citrulline + 2 nitric oxide + 3 oxidized [flavodoxin] (according to UniProt)

Protein family

• NOS family (single member, according to UniProt)

Cofactors

• heme b PubMed
• NADPH PubMed
• FMN or FAD PubMed

Structure

• 2AMO (PDB) (loose dimer),  1M7V (PDB) (bound to tetrahydrofolate and arginine)
• O34453 (AlphaFold)

Expression and Regulation

Operons

• Genes: nos-yflK

  Description: PubMed

  Additional information

  • Note that the yflL is located between nos and yflK, but on the opposite strand

Biological materials

Mutant

• MGNA-C254 (yflM::erm), available at the NBRP B. subtilis, Japan
• BKE07630 (Δnos::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACAAACTCTCCCCTTCAC,  downstream forward: _UP4_AACTATTTTTATCAAGATAA
• BKK07630 (Δnos::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACAAACTCTCCCCTTCAC,  downstream forward: _UP4_AACTATTTTTATCAAGATAA

References

Reviews

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Original Publications

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