TenA

• Description: thiaminase II
  
  

Gene name	tenA
Synonyms
Essential	no
Product	thiaminase II
Function	thiamine salvage
Metabolic function and regulation of this protein in SubtiPathways: Thiamin
MW, pI	27 kDa, 4.99
Gene length, protein length	708 bp, 236 aa
Immediate neighbours	yjbQ, tenI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU11650

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biosynthesis of cofactors

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Thiamine + H₂O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole (according to Swiss-Prot)

• Protein family: thiaminase-2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1YAF, 2QCX (complex with formyl aminommethyl pyrimidine)

• UniProt: P25052

• KEGG entry: [3]

• E.C. number: 3.5.99.2

Expression and regulation

• Operon: tenA-tenI-thiO-thiS-thiG-thiF-thiD PubMed

• Sigma factor:

• Regulation:
  • repressed by thiamine (Thi-box) PubMed

• Regulatory mechanism:
  • Thi-box: RNA switch PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

T P Begley, D M Downs, S E Ealick, F W McLafferty, A P Van Loon, S Taylor, N Campobasso, H J Chiu, C Kinsland, J J Reddick, J Xi
Thiamin biosynthesis in prokaryotes.
Arch Microbiol: 1999, 171(5);293-300
[PubMed:10382260] [WorldCat.org] [DOI] (P p)

Original publications

Amy L Jenkins, Yang Zhang, Steven E Ealick, Tadhg P Begley
Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis.
Bioorg Chem: 2008, 36(1);29-32
[PubMed:18054064] [WorldCat.org] [DOI] (I p)

Amy Haas Jenkins, Ghislain Schyns, Sébastien Potot, Guangxing Sun, Tadhg P Begley
A new thiamin salvage pathway.
Nat Chem Biol: 2007, 3(8);492-7
[PubMed:17618314] [WorldCat.org] [DOI] (P p)

Narasimhan Sudarsan, Smadar Cohen-Chalamish, Shingo Nakamura, Gail Mitchell Emilsson, Ronald R Breaker
Thiamine pyrophosphate riboswitches are targets for the antimicrobial compound pyrithiamine.
Chem Biol: 2005, 12(12);1325-35
[PubMed:16356850] [WorldCat.org] [DOI] (P p)

Angela V Toms, Amy L Haas, Joo-Heon Park, Tadhg P Begley, Steven E Ealick
Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II.
Biochemistry: 2005, 44(7);2319-29
[PubMed:15709744] [WorldCat.org] [DOI] (P p)