RapF

• Description: response regulator aspartate phosphatase, controls ComA activity
  
  

Gene name	rapF
Synonyms	ywhJ
Essential	no
Product	response regulator aspartate phosphatase
Function	control of ComA activity
MW, pI	45 kDa, 5.18
Gene length, protein length	1143 bp, 381 aa
Immediate neighbours	ywhK, phrF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU37460

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

genetic competence, protein modification, transcription factors and their control, quorum sensing

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: control of ComA activity PubMed

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: binding of PhrF to RapF prevents it from binding ComA PubMed

• Interactions: RapF-ComA PubMed, RapF-PhrF PubMed

• Localization:

Database entries

• Structure:

• UniProt: P71002

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapF-phrF PubMed

• Sigma factor:

• Regulation: repressed by glucose (5-fold) (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • ComA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: