GlgB

• Description: 1,4-alpha-glucan branching enzyme
  
  

Gene name	glgB
Synonyms
Essential	no
Product	1,4-alpha-glucan branching enzyme
Function	glycogen biosynthesis
MW, pI	73 kDa, 6.237
Gene length, protein length	1881 bp, 627 aa
Immediate neighbours	glgC, trnB-Glu
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU30980

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

miscellaneous metabolic pathways, sporulation proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 13 family (according to Swiss-Prot)

• Paralogous protein(s): AmyX

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P39118

• KEGG entry: [3]

• E.C. number: 2.4.1.18

Expression and regulation

• Operon: glgB-glgC-glgD-glgA-glgP PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

J A Kiel, J M Boels, G Beldman, G Venema
Glycogen in Bacillus subtilis: molecular characterization of an operon encoding enzymes involved in glycogen biosynthesis and degradation.
Mol Microbiol: 1994, 11(1);203-18
[PubMed:8145641] [WorldCat.org] [DOI] (P p)