EcsA

• Description: ABC transporter (ATP-binding protein), mutation affects proteolysis by RasP (no processing of RsiW and FtsL)
  
  

Gene name	ecsA
Synonyms	prsT, ecs-26, yhaD
Essential	no
Product	ABC transporter (ATP-binding protein)
Function	regulation of the secretion apparatus and of intra-membrane proteolysis
MW, pI	27 kDa, 5.635
Gene length, protein length	741 bp, 247 aa
Immediate neighbours	hit, ecsB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU10040

Phenotypes of a mutant

no processing of RsiW and FtsL PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

ABC transporters, protein secretion, sigma factors and their control, membrane proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ABC transporter domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: EcsA-EcsB PubMed

• Localization: membrane associated (via EcsB) PubMed

Database entries

• Structure:

• UniProt: P55339

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Janine Heinrich, Tuula Lundén, Vesa P Kontinen, Thomas Wiegert
The Bacillus subtilis ABC transporter EcsAB influences intramembrane proteolysis through RasP.
Microbiology (Reading): 2008, 154(Pt 7);1989-1997
[PubMed:18599827] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

S Leskelä, E Wahlström, H L Hyyryläinen, M Jacobs, A Palva, M Sarvas, V P Kontinen
Ecs, an ABC transporter of Bacillus subtilis: dual signal transduction functions affecting expression of secreted proteins as well as their secretion.
Mol Microbiol: 1999, 31(2);533-43
[PubMed:10027970] [WorldCat.org] [DOI] (P p)

Soile Leskelä, Vesa P Kontinen, Matti Sarvas
Molecular analysis of an operon in Bacillus subtilis encoding a novel ABC transporter with a role in exoprotein production, sporulation and competence.
Microbiology (Reading): 1996, 142 ( Pt 1);71-77
[PubMed:8581172] [WorldCat.org] [DOI] (P p)