hit

168

Hit-like protein involved in cell-cycle regulation, similar to Ap₄A hydrolase

locus: BSU_10030

Molecular weight: 16.16 kDa

pI: 6.13

Protein length: 145 aa

Gene length: 438 bp

function: cell-cycle regulation

product: Hit-like protein involved in cell-cycle regulation

essential: no

synonyms: hit, yhaE

List of homologs in different organisms, belongs to COG0537 (Galperin et al., 2021)

Gene

Coordinates: 1,076,515 → 1,076,952

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The protein

[wiki|Domains]

• HIT domain (aa 8-116) (according to UniProt)

Structure

• [PDB|1Y23]
• [AF|O07513]

Expression and Regulation

Operons

• genes: [gene|F8E5A679BB15E5714A5EBE5FF001429D6FFA8B66|hit]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A688 (hit::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/688 NBRP B. subtilis, Japan]
• BKE10030 (Δ[gene|F8E5A679BB15E5714A5EBE5FF001429D6FFA8B66|hit]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10030 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAGGGTTCCTCCTTATG,  downstream forward: _UP4_TAAGATGAGGTTATTTTATT
• BKK10030 (Δ[gene|F8E5A679BB15E5714A5EBE5FF001429D6FFA8B66|hit]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10030 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAGGGTTCCTCCTTATG,  downstream forward: _UP4_TAAGATGAGGTTATTTTATT