Phy

• Description: phytase
  
  

Gene name	phy
Synonyms	yodV, yzxA
Essential	no
Product	3-phytase
Function	untilization of inositol hexakisphosphate (phytate)
MW, pI	41 kDa, 4.936
Gene length, protein length	1146 bp, 382 aa
Immediate neighbours	cgeB, yodU
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU19800

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Myo-inositol hexakisphosphate + H₂O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate (according to Swiss-Prot)

• Protein family: msrA Met sulfoxide reductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure: 1QLG (enzyme from B. amyloliquefaciens, 72% identity)

• UniProt: P42094

• KEGG entry: [3]

• E.C. number: 3.1.3.8

Expression and regulation

• Operon: phy PubMed

• Sigma factor: none PubMed

• Regulation:

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:
  • the gene is not expressed in B. subtilis due to the absene of a functional promoter PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: