SufS

• Description: cysteine desulfurase
  
  

Gene name	sufS
Synonyms	yurW, csd
Essential	yes PubMed
Product	cysteine desulfurase
Function	formation of iron-sulfur clusters in proteins
MW, pI	44 kDa, 5.199
Gene length, protein length	1218 bp, 406 aa
Immediate neighbours	sufU, sufD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU32690

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine (according to Swiss-Prot)

• Protein family: Csd subfamily (according to Swiss-Prot)

• Paralogous protein(s): NifS, YrvO, NifZ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: interaction with SufU enhances the activity of SufS 40-fold PubMed

• Interactions: SufS-SufU PubMed

• Localization:

Database entries

• Structure:

• UniProt: O32164

• KEGG entry: [2]

• E.C. number: 2.8.1.7

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Bruna Selbach, Emily Earles, Patricia C Dos Santos
Kinetic analysis of the bisubstrate cysteine desulfurase SufS from Bacillus subtilis.
Biochemistry: 2010, 49(40);8794-802
[PubMed:20822158] [WorldCat.org] [DOI] (I p)

Alexander G Albrecht, Daili J A Netz, Marcus Miethke, Antonio J Pierik, Olaf Burghaus, Florian Peuckert, Roland Lill, Mohamed A Marahiel
SufU is an essential iron-sulfur cluster scaffold protein in Bacillus subtilis.
J Bacteriol: 2010, 192(6);1643-51
[PubMed:20097860] [WorldCat.org] [DOI] (I p)