Difference between revisions of "BioI"
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− | <pubmed> 18838690 ,8763940,8892842,15449931, 15449930 12538057 20658980 11368323 </pubmed> | + | <pubmed> 18838690 ,8763940,8892842,15449931, 15449930 12538057 20658980 11368323 11472016 14737344 12943422 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 15:11, 28 July 2010
- Description: cytochrome P450 enzyme
Gene name | bioI |
Synonyms | |
Essential | no |
Product | cytochrome P450 enzyme |
Function | biosynthesis of biotin |
MW, pI | 44 kDa, 6.2 |
Gene length, protein length | 1185 bp, 395 aa |
Immediate neighbours | ytbQ, bioB |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context ![]() This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU30190
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: formation of pimelic acid via a multiple-step oxidative cleavage of long-chain fatty acids
- Protein family: cytochrome P450 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- UniProt: P53554
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Max J Cryle
Selectivity in a barren landscape: the P450(BioI)-ACP complex.
Biochem Soc Trans: 2010, 38(4);934-9
[PubMed:20658980]
[WorldCat.org]
[DOI]
(I p)
Max J Cryle, Ilme Schlichting
Structural insights from a P450 Carrier Protein complex reveal how specificity is achieved in the P450(BioI) ACP complex.
Proc Natl Acad Sci U S A: 2008, 105(41);15696-701
[PubMed:18838690]
[WorldCat.org]
[DOI]
(I p)
Rachel J Lawson, David Leys, Michael J Sutcliffe, Carol A Kemp, Myles R Cheesman, Susan J Smith, John Clarkson, W Ewen Smith, Ihtshamul Haq, John B Perkins, Andrew W Munro
Thermodynamic and biophysical characterization of cytochrome P450 BioI from Bacillus subtilis.
Biochemistry: 2004, 43(39);12410-26
[PubMed:15449931]
[WorldCat.org]
[DOI]
(P p)
Rachel J Lawson, Claes von Wachenfeldt, Ihtshamul Haq, John Perkins, Andrew W Munro
Expression and characterization of the two flavodoxin proteins of Bacillus subtilis, YkuN and YkuP: biophysical properties and interactions with cytochrome P450 BioI.
Biochemistry: 2004, 43(39);12390-409
[PubMed:15449930]
[WorldCat.org]
[DOI]
(P p)
Max J Cryle, James J De Voss
Carbon-carbon bond cleavage by cytochrome p450(BioI)(CYP107H1).
Chem Commun (Camb): 2004, (1);86-7
[PubMed:14737344]
[WorldCat.org]
[DOI]
(P p)
Max J Cryle, Nick J Matovic, James J De Voss
Products of cytochrome P450(BioI) (CYP107H1)-catalyzed oxidation of fatty acids.
Org Lett: 2003, 5(18);3341-4
[PubMed:12943422]
[WorldCat.org]
[DOI]
(P p)
Amanda J Green, Andrew W Munro, Myles R Cheesman, Graeme A Reid, Claes von Wachenfeldt, Stephen K Chapman
Expression, purification and characterisation of a Bacillus subtilis ferredoxin: a potential electron transfer donor to cytochrome P450 BioI.
J Inorg Biochem: 2003, 93(1-2);92-9
[PubMed:12538057]
[WorldCat.org]
[DOI]
(P p)
A J Green, S L Rivers, M Cheeseman, G A Reid, L G Quaroni, I D Macdonald, S K Chapman, A W Munro
Expression, purification and characterization of cytochrome P450 Biol: a novel P450 involved in biotin synthesis in Bacillus subtilis.
J Biol Inorg Chem: 2001, 6(5-6);523-33
[PubMed:11472016]
[WorldCat.org]
[DOI]
(P p)
J E Stok, J De Voss
Expression, purification, and characterization of BioI: a carbon-carbon bond cleaving cytochrome P450 involved in biotin biosynthesis in Bacillus subtilis.
Arch Biochem Biophys: 2000, 384(2);351-60
[PubMed:11368323]
[WorldCat.org]
[DOI]
(P p)
J B Perkins, S Bower, C L Howitt, R R Yocum, J Pero
Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis.
J Bacteriol: 1996, 178(21);6361-5
[PubMed:8892842]
[WorldCat.org]
[DOI]
(P p)
S Bower, J B Perkins, R R Yocum, C L Howitt, P Rahaim, J Pero
Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.
J Bacteriol: 1996, 178(14);4122-30
[PubMed:8763940]
[WorldCat.org]
[DOI]
(P p)