Difference between revisions of "MtlR"

From SubtiWiki
Jump to: navigation, search
Line 1: Line 1:
* '''Description:''' transcriptional activator of the ''[[mtlA]]-[[mtlD]]'' operon <br/><br/>
+
* '''Description:''' transcriptional activator of the ''[[mtlA]]-[[mtlF]]-[[mtlD]]'' operon <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
Line 64: Line 64:
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''Domains:''' N-terminal DNA-binding domains, two [[PTS]]-regulation domains (PRD1 and PRD2), EIIB (Gat)-like domain, EIIA (Mtl)-like domain
  
* '''Modification:'''
+
* '''Modification:'''  
 +
** phosphorylated on His-342 and/or His-399 by [[PtsH]] {{PubMed|20444094}}
 +
** phosphorylation on His-599 by [[MtlA]] {{PubMed|20444094}}
 +
** phosphorylation on Cys-419 by [[MtlF]] {{PubMed|20444094}}
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
 +
** activity is stimulated by [[PtsH]]-dependent phosphorylation in PRD2 (mechnism of carbon catabolite repression) {{PubMed|20444094}}
 +
** activity is inhibited by [[MtlF]]-dependent phosphorylation in the EIIB(Gat)-like domain on Cys-419 (this prevents activity in the absence of mannitol and allows induction in presence of mannitol) {{PubMed|20444094}}
  
* '''Interactions:''' [[PtsH]]-[[MtlR]],  [[MtlA]]-[[MtlR]]
+
* '''Interactions:''' [[PtsH]]-[[MtlR]] {{PubMed|20444094}},  [[MtlA]]-[[MtlR]] {{PubMed|20444094}},  [[MtlF]]-[[MtlR]] {{PubMed|20444094}}
  
 
* '''Localization:'''
 
* '''Localization:'''
Line 120: Line 125:
 
=References=
 
=References=
  
<pubmed>12897001,,10627040, </pubmed>
+
<pubmed>12897001, 20444094,10627040, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 13:08, 11 May 2010

  • Description: transcriptional activator of the mtlA-mtlF-mtlD operon

Gene name mtlR
Synonyms ydaA
Essential no
Product transcriptional activator, PRD-type
Function regulation of mannitol utilization
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 78 kDa, 5.313
Gene length, protein length 2082 bp, 694 aa
Immediate neighbours ycsN, ydaB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MtlR context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU04160

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: N-terminal DNA-binding domains, two PTS-regulation domains (PRD1 and PRD2), EIIB (Gat)-like domain, EIIA (Mtl)-like domain
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • activity is stimulated by PtsH-dependent phosphorylation in PRD2 (mechnism of carbon catabolite repression) PubMed
    • activity is inhibited by MtlF-dependent phosphorylation in the EIIB(Gat)-like domain on Cys-419 (this prevents activity in the absence of mannitol and allows induction in presence of mannitol) PubMed
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References