Difference between revisions of "EcsA"
| Line 1: | Line 1: | ||
| − | * '''Description:''' ABC transporter (ATP-binding protein), mutation affects proteolysis by RasP (no processing of RsiW and FtsL) <br/><br/> | + | * '''Description:''' [[ABC transporter]] (ATP-binding protein), mutation affects proteolysis by [[RasP]] (no processing of [[RsiW]] and [[FtsL]]) <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 10: | Line 10: | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || ABC transporter (ATP-binding protein) | + | |style="background:#ABCDEF;" align="center"| '''Product''' || [[ABC transporter]] (ATP-binding protein) |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of | + | |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of the secretion apparatus and of intra-membrane proteolysis |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.635 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.635 | ||
| Line 54: | Line 54: | ||
* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
| − | * '''Protein family:''' ABC transporter domain (according to Swiss-Prot) | + | * '''Protein family:''' [[ABC transporter]] domain (according to Swiss-Prot) |
* '''Paralogous protein(s):''' | * '''Paralogous protein(s):''' | ||
| Line 70: | Line 70: | ||
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' | + | * '''Interactions:''' [[EcsA]]-[[EcsB]] {{PubMed|10092453}} |
* '''Localization:''' | * '''Localization:''' | ||
Revision as of 20:20, 13 January 2010
- Description: ABC transporter (ATP-binding protein), mutation affects proteolysis by RasP (no processing of RsiW and FtsL)
| Gene name | ecsA |
| Synonyms | prsT, ecs-26, yhaD |
| Essential | no |
| Product | ABC transporter (ATP-binding protein) |
| Function | regulation of the secretion apparatus and of intra-membrane proteolysis |
| MW, pI | 27 kDa, 5.635 |
| Gene length, protein length | 741 bp, 247 aa |
| Immediate neighbours | hit, ecsB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU10040
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: ABC transporter domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- UniProt: P55339
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information: the mRNA is very stable (half-life > 15 min) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Janine Heinrich, Tuula Lundén, Vesa P Kontinen, Thomas Wiegert
The Bacillus subtilis ABC transporter EcsAB influences intramembrane proteolysis through RasP.
Microbiology (Reading): 2008, 154(Pt 7);1989-1997
[PubMed:18599827]
[WorldCat.org]
[DOI]
(P p)
G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008]
[WorldCat.org]
[DOI]
(P p)
Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453]
[WorldCat.org]
[DOI]
(P p)
S Leskelä, E Wahlström, H L Hyyryläinen, M Jacobs, A Palva, M Sarvas, V P Kontinen
Ecs, an ABC transporter of Bacillus subtilis: dual signal transduction functions affecting expression of secreted proteins as well as their secretion.
Mol Microbiol: 1999, 31(2);533-43
[PubMed:10027970]
[WorldCat.org]
[DOI]
(P p)
Soile Leskelä, Vesa P Kontinen, Matti Sarvas
Molecular analysis of an operon in Bacillus subtilis encoding a novel ABC transporter with a role in exoprotein production, sporulation and competence.
Microbiology (Reading): 1996, 142 ( Pt 1);71-77
[PubMed:8581172]
[WorldCat.org]
[DOI]
(P p)
