UvrC

• Description: excinuclease ABC (subunit C)
  
  

Gene name	uvrC
Synonyms	uvrB
Essential	no
Product	excinuclease ABC (subunit C)
Function	DNA repair after UV damage
MW, pI	69 kDa, 9.503
Gene length, protein length	1794 bp, 598 aa
Immediate neighbours	ask, trxA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU28490

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: uvrC family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: UvrB-UvrC PubMed

• Localization:

Database entries

• Structure: 3C65 (5' endonuclease domain, Geobacillus stearothermophilus)

• UniProt: P14951

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: uvrC PubMed

• Sigma factor:

• Regulation:
  • induced upon DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed]

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

James J Truglio, Deborah L Croteau, Bennett Van Houten, Caroline Kisker
Prokaryotic nucleotide excision repair: the UvrABC system.
Chem Rev: 2006, 106(2);233-52
[PubMed:16464004] [WorldCat.org] [DOI] (P p)

Bennett Van Houten, Deborah L Croteau, Matthew J DellaVecchia, Hong Wang, Caroline Kisker
'Close-fitting sleeves': DNA damage recognition by the UvrABC nuclease system.
Mutat Res: 2005, 577(1-2);92-117
[PubMed:15927210] [WorldCat.org] [DOI] (P p)

A Sancar
Mechanisms of DNA excision repair.
Science: 1994, 266(5193);1954-6
[PubMed:7801120] [WorldCat.org] [DOI] (P p)

Original Publications

Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290] [WorldCat.org] [DOI] (P p)

J J Lin, A Sancar
Reconstitution of nucleotide excision nuclease with UvrA and UvrB proteins from Escherichia coli and UvrC protein from Bacillus subtilis.
J Biol Chem: 1990, 265(34);21337-41
[PubMed:2174442] [WorldCat.org] (P p)

N Y Chen, J J Zhang, H Paulus
Chromosomal location of the Bacillus subtilis aspartokinase II gene and nucleotide sequence of the adjacent genes homologous to uvrC and trx of Escherichia coli.
J Gen Microbiol: 1989, 135(11);2931-40
[PubMed:2559145] [WorldCat.org] [DOI] (P p)