Difference between revisions of "GlsA"
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[glsA]]-[[glnT]]'' {{PubMed|15995196}} |
| − | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|15995196}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** induced in the presence of glutamine ([[GlnL]]) {{PubMed|15995196}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[GlnL]]: transcription activation {{PubMed|15995196}} | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 09:48, 2 August 2009
- Description: glutaminase, low affinity for glutamine
| Gene name | glsA |
| Synonyms | ybgJ |
| Essential | no |
| Product | glutaminase |
| Function | glutamine degradation |
| Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate | |
| MW, pI | 36 kDa, 5.315 |
| Gene length, protein length | 981 bp, 327 aa |
| Immediate neighbours | glnT, glnK |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU02430
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: l-glutamine + H2O = L-glutamate + NH3 (according to Swiss-Prot)
- Protein family: glutaminase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 3BRM
- UniProt: O31465
- KEGG entry: [3]
- E.C. number: 3.5.1.2
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Greg Brown, Alex Singer, Michael Proudfoot, Tatiana Skarina, Youngchang Kim, Changsoo Chang, Irina Dementieva, Ekaterina Kuznetsova, Claudio F Gonzalez, Andrzej Joachimiak, Alexei Savchenko, Alexander F Yakunin
Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis.
Biochemistry: 2008, 47(21);5724-35
[PubMed:18459799]
[WorldCat.org]
[DOI]
(I p)
Takenori Satomura, Daisuke Shimura, Kei Asai, Yoshito Sadaie, Kazutake Hirooka, Yasutaro Fujita
Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system.
J Bacteriol: 2005, 187(14);4813-21
[PubMed:15995196]
[WorldCat.org]
[DOI]
(P p)
