RNA polymerase

The enzyme responsible for [wiki|transcription]

As an important difference as compared to the E. coli enzyme, the B. subtilis RNA polymerase has a strong preference for G as first nucleotides in transcripts PubMed

Components of the RNA polymerase

Core subunits

• [protein|5996A5C25E108A3C4562686BF34A59CB14FD56EB|rpoA]: alpha subunit
• [protein|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB]: beta subunit
• [protein|BC8B37355E6FADBA5F4636C3879E99003B0F3E84|rpoC]: beta' subunit

• The stoichiometry of the enzyme is [protein|5996A5C25E108A3C4562686BF34A59CB14FD56EB|rpoA](2)-[protein|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB]-[protein|BC8B37355E6FADBA5F4636C3879E99003B0F3E84|rpoC]

[wiki|Sigma factors]

• In addition to the housekeeping [wiki|sigma factor], [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA], there are several other [protein|search|sigma factors] with different promoter recognition specifiity that are active under specific conditions (such as stress or sporulation)

Small accessory subunits

• [protein|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]: delta subunit
• [protein|5F41273FB867C688011738CD45AA6FF5D3987EFE|rpoY]: epsilon subunit
• [protein|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]: omega subunit

Other interaction partners

• [protein|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|nusA]: essential elongation factor
• [protein|6FBF27C12B4B3DC2CE373E5199357042114471F8|greA]: resolves promoter proximal pausing of RNA polymerase PubMed
• [protein|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|cshA]: [wiki|DEAD-box RNA helicase] PubMed
• [protein|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA]: ATP-dependent DNA helicase PubMed

Temporary interaction partners

• [protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: transcription regulator, interacts with [protein|5996A5C25E108A3C4562686BF34A59CB14FD56EB|rpoA]
• [protein|6A38DAA96F7BBF31FD8A4018A8CA7A72F3C28F79|mgsR]: transcription regulator orthologous to [protein|2C6386E9A63F410558D168798D077DF91590F454|spx], interacts with [protein|5996A5C25E108A3C4562686BF34A59CB14FD56EB|rpoA]
• [protein|EB28A65CECE994DF2DF486DEACF40F2533703DB0|btr]: transcription activator PubMed
• [protein|8AB50581EBB3C27BB9A96EA3382F8C90CE5B6EC5|ylyA]: modulates [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]-dependent transcription PubMed

Additional interaction partners of the RNA polymerase (no specific subunit specified)

[protein|4DE90422754DA1FD9EFE58766EACB99CE6171207|helD], [protein|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA], [protein|A40CD2C23A19860342F440284302EFFDAC09E88A|cssR], [protein|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|rnhC], [protein|0DF27BEAF763545258CE7DD56F65CD5BF74DD887|ypsC], [protein|AD1F39823ABCE2A222259B879513752942C59590|mfd], [protein|057BFF91609C918CEE4E6FF9100A1E4959BF6715|ypiA], [protein|1F845CE702BCD10F4DB596E438CE9DB076806B1D|ydjO], [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD] PubMed

References

Reviews

<pubmed> 18599813 7708009 18410247 19889534 19489723 18280161 21233849 22210308 23433801 23768203 24763425 25878038 26132790 26010401 16524917,28657884,29856930</pubmed>

The structure of RNA polymerase

<pubmed> 12732296 12581657 11297923 19735077 26293966 28652344</pubmed>

Important original publications

<pubmed> 21350489 21515770 22333917,20817769,20724389,21710567 23761441 23875654 23771146 24789973 25961799 26400263 27622946 27977677 29454936 </pubmed>