sigE

sigE
168

RNA polymerase sporulation mother cell-specific (early) sigma factor SigE

Locus
BSU_15320
Molecular weight
27.55 kDa
Isoelectric point
9.21
Protein length
239 aaSequenceBlast
Gene length
720 bpSequenceBlast
Function
transcription of sporulation genes (early mother cell)
Product
RNA polymerase sporulation mother cell-specific (early) sigma factor SigE
Essential
no
Synonyms
sigE, spoIIGB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5811 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,604,771  1,605,490
The protein
Catalyzed reaction/ biological activity
RNA polymerase sigma factor, active in the mother cell
Protein family
Sigma-70 factor family (according to UniProt)
Structure
8B3Z (PDB) (aa 17 ... 133) PubMed
1L0O (PDB) (SigF in complex with SpoIIAB, Geobacillus stearothermophilus, 33% identity) PubMed
P06222 (AlphaFold)
Expression and Regulation
Operons
Genes
spoIIGA-sigE-sigG
Description
PubMed
Regulation
''spoIIGA'': expressed under conditions that trigger sporulation (Spo0A) PubMed
Regulatory mechanism
SinR: repression, in sinR regulon
Spo0A: activation, PubMed, in spo0A regulon
AbrB: repression, PubMed, in abrB regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigG: sigma factor, PubMed, in sigG regulon
SigF: sigma factor, in sigF regulon
Additional information
the mRNA half-life is about 2.6 min PubMed
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spoIIGA→sigG

2025-04-02 13:09:40

ghost

142

3354b759a5d3c11fb5b0d40216c1a7e1d8249a30

020EF37B0706DBB0AF1DC411F77E0B6D94001319

Additional information
translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
Biological materials
Mutant
BKE15320 (sigE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTCCTCTCCCTTCTAA,  downstream forward: _UP4_TAAAAAATTTTATGGTTAGA
BKK15320 (sigE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTCCTCTCCCTTCTAA,  downstream forward: _UP4_TAAAAAATTTTATGGTTAGA
Labs working on this gene/protein
Bill Haldenwang, San Antonio, USA
Charles Moran, Emory University, NC, USA homepage
References
Reviews
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Original Publications
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The SigE regulon
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3CB24D493B3282278B7DC61CE3C793DFA815F9E2

Page visits: 10679

Time of last update: 2025-04-05 16:40:33

Author of last update: Jstuelk