pyrR
168
transcriptional termination factor of the pyr operon
locus
BSU_15470
Molecular weight
20.12 kDa
pI
4.99
function
regulation of pyrimidine biosynthesis
product
transcriptional termination protein with minor uracil phosphoribosyltransferase activity
essential
no
ec
2.4.2.9
synonyms
pyrR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2065 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,618,304 1,618,849
The protein
Catalyzed reaction/ biological activity
diphosphate + UMP --> 5-phospho-α-D-ribose 1-diphosphate + uracil (according to UniProt)
Protein family
[wiki|Purine/pyrimidine phosphoribosyltransferase family] (according to UniProt)
Structure
[PDB|1A3C] (dimeric form)
[AF|P39765]
Effectors of protein activity
UMP and UTP stimulate the RNA-binding activity of PyrR, resulting of transcription termination of the [gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]-[gene|A5C26E10085868A0A193C450DF58D54C636B7C2D|pyrP]-[gene|B83462A50FDDD20FE9BD4681824E30AAD10F506B|pyrB]-[gene|CA16FD3507DCCADB1E01DF25B42C3405C9205B05|pyrC]-[gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]-[gene|D57DC8A53E52BC4A2EA894E3C9FD983592882918|pyrAB]-[gene|C20A05AE4CE8AEE0DA767334935CC64106A7FCCB|pyrK]-[gene|E533BEF28579CD04B24E2C728737269E35224450|pyrD]-[gene|5D0D0D2413377FCA92440334E92B5A27A5963D9E|pyrF]-[gene|0197A037D2AF049B295E1DA60187D4614B2BAC51|pyrE] operon [pubmed|31100987]
Expression and Regulation
Operons
genes
[gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]-[gene|A5C26E10085868A0A193C450DF58D54C636B7C2D|pyrP]-[gene|B83462A50FDDD20FE9BD4681824E30AAD10F506B|pyrB]-[gene|CA16FD3507DCCADB1E01DF25B42C3405C9205B05|pyrC]-[gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]-[gene|D57DC8A53E52BC4A2EA894E3C9FD983592882918|pyrAB]-[gene|C20A05AE4CE8AEE0DA767334935CC64106A7FCCB|pyrK]-[gene|E533BEF28579CD04B24E2C728737269E35224450|pyrD]-[gene|5D0D0D2413377FCA92440334E92B5A27A5963D9E|pyrF]-[gene|0197A037D2AF049B295E1DA60187D4614B2BAC51|pyrE]
description
[Pubmed|8206849]
regulation
induced in the absence of uridine nucleotides ([protein|search|PyrR]) [Pubmed|8206849]
regulatory mechanism
[protein|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]: termination, via [wiki|RNA switch], in [regulon|protein:6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1709162], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]
description
[Pubmed|22383849]
genes
[gene|8FC1A0A6C9975F6C125490FBAAB2A4957210A9F1|lspA]-[gene|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|rluD]-[gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]
description
[Pubmed|22383849]
Biological materials
Mutant
BKE15470 ([gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTGTGACACCTCACAG, downstream forward: _UP4_GCCATTTATGAAAACGAATA
BKK15470 ([gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTGTGACACCTCACAG, downstream forward: _UP4_GCCATTTATGAAAACGAATA
References
Reviews
Original Publications
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Time of last update: 2025-04-07 02:06:55
Author of last update: Jstuelk