dprA
168
conveys incoming ssDNA to [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]; facilitates the displacement of both SSBs ([protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]), increases [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] nucleation onto SSB-coated ssDNA, and mediates DNA strand annealing, required to internalize and to recombine ssDNA with homologous resident duplex, crucial for the [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-dependent acquisition of homologous genes from related species by natural transformation
locus
BSU_16110
Molecular weight
32.78 kDa
pI
9.76
function
[category|SW.3.1.5|DNA repair/ recombination]
product
recombination mediator protein
essential
no
synonyms
dprA, smf
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0758 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,682,580 → 1,683,473
Phenotypes of a mutant
strongly impaired genetic recombination [Pubmed|23779106]
''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]'' double mutants have a strongly reduced chromosomal transformation rate [Pubmed|23779106,22373918]
loss of plasmid transformation [pubmed|30050509]
The protein
Catalyzed reaction/ biological activity
binds ssDNA, promotes displacement of [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA] and [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB] from ssDNA [Pubmed|23779106]
provides [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] access to ssDNA during chromosomal transformation (together with [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]) [Pubmed|22373918]
[protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-ATP in concert with [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA] catalyzes DNA strand exchange, with [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB] as an accessory factor [Pubmed|25138221]
the [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA] mediator loads [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] onto any fragmented linear SPP1 ssDNA [pubmed|31876108]
anneals [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]- or [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB]-coated complementary strands of transfecting SPP1 phage DNA, yielding tailed SPP1 duplex intermediates [pubmed|31876108]
[protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA], [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] or viral single strand annealing G35P protein, may catalyze the annealing of complete linear phage genomes with redundant regions at the ends of the molecule, alone or with the help of an exonuclease, to produce a circular unit-length duplex viral genome ready to initiate replication [pubmed|31876108]
Protein family
DprA/Smf family (single member, according to UniProt)
Structure
[PDB|3UQZ] (aa 90 ... 310, from Streptococcus pneumoniae, 44% identity) [pubmed|22904190]
[AF|P39813]
[wiki|Localization]
transiently localizes to the cell pole, and co-localizes with the DNA uptake machinery [Pubmed|17630974]
Expression and Regulation
Operons
genes
[gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD]-[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]-[gene|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA]
description
[Pubmed|22383849]
regulation
repressed by glucose (2.4-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|12850135]
expression is heterogeneous [pubmed|29809139]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
additional information
expressed under conditions that trigger genetic competence ([protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]) [Pubmed|11948146]
Biological materials
Mutant
BKE16110 (Δ[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATAGATTGACCTCCTTTT, downstream forward: _UP4_TGAATTATCGTTTGACAAAC
BKK16110 (Δ[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16110 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CAATAGATTGACCTCCTTTT, downstream forward: _UP4_TGAATTATCGTTTGACAAAC
labs
[wiki|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]
References
Reviews
Original Publications
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Time of last update: 2025-04-02 18:28:14
Author of last update: Jstuelk