desR
168
two-component response regulator, regulation of cold shock expression of [gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]
locus
BSU_19200
Molecular weight
22.03 kDa
pI
4.89
function
regulation of cold shock expression of [gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]
product
two-component response regulator
essential
no
synonyms
desR, yocG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2197 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,091,705 → 2,092,304
The protein
Catalyzed reaction/ biological activity
transcription activation of the ''[gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]'' operon when phosphorylated by [protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]
[wiki|Domains]
[wiki|Response regulatory domain] (aa 3-117) (according to UniProt)
[wiki|HTH luxR-type domain] (aa 131-196) (according to UniProt)
Structure
[PDB|5IUJ] ([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]-[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR] complex in the phosphotransfer state with low Mg2+ [20 mM]) [Pubmed|27938660]
[PDB|5IUK] ([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]-[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR] complex in the phosphotransfer state with high Mg2+ [150 mM] and BeF3) [Pubmed|27938660]
[PDB|5IUN] ([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]-[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR] complex in the phosphatase state) [Pubmed|27938660]
[AF|O34723]
Modification
phosphorylated by [protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK] on an Asp residue
Paralogous protein(s)
[protein|5BB2B90D5DDD50DD9B4EF9E69925FCBFA642222B|yvfU]
Expression and Regulation
Operons
genes
[gene|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]-[gene|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR]
description
[Pubmed|11285232]
regulation
induced by cold shock (12-fold) [Pubmed|12399512]
Biological materials
Mutant
MGNA-A320 (yocG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/320 NBRP B. subtilis, Japan]
BKE19200 (Δ[gene|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCATATAAGCCATCCTTT, downstream forward: _UP4_TAAAAAAGGATCTTGGCATC
BKK19200 (Δ[gene|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCATATAAGCCATCCTTT, downstream forward: _UP4_TAAAAAAGGATCTTGGCATC
labs
[wiki|Diego de Mendoza], Universidad Nacional de Rosario, Argentine [http://www.ibr.gov.ar/eng/investigacion/demendoza.htm homepage]
References
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Time of last update: 2024-12-22 08:31:19
Author of last update: Jstuelk