gerKA
168
nutrient receptor
locus
BSU_03700
Molecular weight
60.22 kDa
pI
5.21
function
germination response to the combination of glucose, fructose, aspartate, and KCl
product
part of the putative nutrient-gated [protein|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]-[protein|D1AFCB1BE693AB6B8461B7764B6641E6EB1404FA|gerKB]-[protein|D5D4E1E182F972BEF6BB432A7FF25EF45F1F0D95|gerKC] ion channel
essential
no
synonyms
gerKA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5901 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
420,110 → 421,744
The protein
Catalyzed reaction/ biological activity
the [protein|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]-[protein|D1AFCB1BE693AB6B8461B7764B6641E6EB1404FA|gerKB]-[protein|D5D4E1E182F972BEF6BB432A7FF25EF45F1F0D95|gerKC] ion channel likely releases ions from the spore core in response to asparagine, glucose, fructose and K+ to trigger [category|SW.4.2.4|Germination], this requires the gerB locus [pubmed|37104613]
Protein family
[wiki|GerABKA family] (according to UniProt)
[wiki|Domains]
contains multiple membrane-spanning domains [Pubmed|23335419]
Structure
[PDB|6O59] (from B. megaterium, corresponds to aa 40 ... 299, 53.6% identity) [pubmed|31113879]
[AF|P49939]
Paralogous protein(s)
[protein|EEDE6A22E91992ABCA677CAB7917B7168C5CB868|gerAA], [protein|AE28299B0F274B189DD90554A5A4F7B9A9479B60|yndD], [protein|3368743E6E03792DB83A38A19989123304DF7560|gerBA], [protein|7CE0CAF27B1C0F507E783B8B80622270B00634FD|yfkQ]
[wiki|Localization]
outer surface of the inner spore membrane [Pubmed|23335419,21696470]
Additional information
700 molecules are present per spore [Pubmed|23749970]
Expression and Regulation
Operons
genes
[gene|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]-[gene|D5D4E1E182F972BEF6BB432A7FF25EF45F1F0D95|gerKC]-[gene|D1AFCB1BE693AB6B8461B7764B6641E6EB1404FA|gerKB]
description
[pubmed|22383849]
regulation
expressed during sporulation ([protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG], [wiki|SpoVT]) [Pubmed|16707705]
regulatory mechanism
[protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]: repression, [Pubmed|16707705], in [regulon|protein:01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT regulon]
sigma factors
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|16707705], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
additional information
700 molecules are present per spore [PubMed|23749970]
Biological materials
Mutant
BKE03700 (Δ[gene|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACACAAATACCTTTCCT, downstream forward: _UP4_AAAGATTTGGAAGAAGGGGA
BKK03700 (Δ[gene|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACACAAATACCTTTCCT, downstream forward: _UP4_AAAGATTTGGAAGAAGGGGA
References
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Time of last update: 2024-12-19 16:44:04
Author of last update: Jstuelk