uvrB
168
excinuclease ABC (subunit B), required for transcription-dependent asymmetry in mutation rates of genes in the two orientations
locus
BSU_35170
Molecular weight
76.15 kDa
pI
5.26
function
DNA repair after UV damage
product
excinuclease ABC (subunit B)
essential
no
synonyms
uvrB, dinA, uvrA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0556 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,612,945 → 3,614,930
Phenotypes of a mutant
a [gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA] [gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB] mutant is sensitive to blue light-induced DNA damage [pubmed|30054368]
reduced resistance towards electron beams [pubmed|31948638]
The protein
Protein family
UvrB family (with [protein|AD1F39823ABCE2A222259B879513752942C59590|mfd], according to UniProt)
[wiki|Domains]
[wiki|Helicase ATP-binding domain] (aa 26-413) (according to UniProt)
[wiki|Helicase C-terminal domain] (aa 430-596) (according to UniProt)
[wiki|UVR domain] (aa 625-660) (according to UniProt)
Structure
[PDB|2NMV] (bound to fluorescein-adducted DNA); [PDB|2D7D] ternary complex involving UvrB* (a C-terminal truncation of full-length UvrB), a polythymine trinucleotide and ADP [Pubmed|16426634]
[AF|P37954]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB]-[gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA]
description
[Pubmed|22383849]
regulation
induced upon DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]) [Pubmed|8226626]
regulatory mechanism
[protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|8226626], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]
additional information
during [protein|search|sporulation] expressed both in the forespore and the mother cell [PubMed|24118570]
expression is induced in the presence of Cr(VI) [pubmed|30745368]
Biological materials
Mutant
GP3534 (Δ([gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA]-[gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB])::kan), available in [wiki|Jörg Stülke]'s lab
GP1175 (Δ([gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA]-[gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB])::ermC), available in [wiki|Jörg Stülke]'s lab [pubmed|22178973]
BKE35170 (Δ[gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAAAATAAGCCTCCGTT, downstream forward: _UP4_CTAAAAGCGGAAGGATGATG
BKK35170 (Δ[gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAAAATAAGCCTCCGTT, downstream forward: _UP4_CTAAAAGCGGAAGGATGATG
References
Reviews
Original Publications
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Time of last update: 2025-04-03 00:53:22
Author of last update: Jstuelk